This study identified plant rhizobacteria that have the capability to degrade polycyclic aromatic hydrocarbons (PAHs) and assist the plant in enhancing the phytoremediation process of PAH-contaminated wastewater. Rhizobacteria were isolated from the rhizosphere zone of Lepironia articulata exposed to 1%, 2% and 3% diesel concentrations (V_Diesel/V_Water). Thirty colonies were isolated, and after morphological and biochemical tests, they were classified into 23 colonies. These colonies were screened with a 3% diesel concentration (V_Diesel/V_Water) and only nine rhizobacteria (coded as A, B, C, D, E, F, G, H and I) were able to tolerate and sustain this. The extensive biodegradation of PAHs was carried out on the nine isolated rhizobacteria (individually as a monoculture), and it was found that the three rhizobacteria identified as Pseudomonas toyotomiensis strain ND1 (E), Microbacterium resistens strain ND2 (G) and Bacillus pumilus train ND3 (I) were highly able to remove PAHs (>80 % removal). Further biodegradation studies on the 16 components of PAHs were conducted for mixed cultures (E + G + I, G + E, G + I and E + I), and a mixed culture of G + E was found to be the best group in degrading PAHs (89 % removal). The existence of rhizobacteria, either as a monoculture or mixed culture, assisted the degradation of PAHs, thus enhancing the removal of PAH from wastewater. This study revealed the promising potential of the newly identified PAH degraders as candidates in enhancing the phytoremediation efficiency of PAH.

这项研究确定了具有降解多环芳烃（PAH）并协助植物增强PAH污染废水的植物修复过程的植物根际细菌。从暴露于1％，2％和3％柴油浓度（V_柴油/ V_水）的Lepironia articulata的根际区分离出根瘤菌。分离出三十个菌落，经过形态和生化测试，将其分为23个菌落。用3％的柴油浓度（V_柴油/ V_水），只有9种根瘤菌（编码为A，B，C，D，E，F，G，H和I）能够耐受并维持这种状态。PAHs在9种分离的根际细菌上进行了广泛的生物降解（单独作为单一培养物），发现这3种根瘤菌被鉴定为丰田假单胞菌ND1菌株（E），抗性微细菌ND2菌株（G）和短小芽孢杆菌。火车ND3（I）能够高度去除PAH（去除率> 80％）。针对混合培养（E + G + I，G + E，G + I和E + I）对PAHs的16种成分进行了进一步的生物降解研究，发现G + E的混合培养是最佳组合。降解多环芳烃（去除89％）。根际细菌的存在，无论是单一培养还是混合培养，都有助于PAHs的降解，从而促进了废水中PAH的去除。这项研究揭示了新发现的PAH降解物作为候选物在增强PAH的植物修复效率方面的潜力。