Non-human leukocyte antigen (HLA) antibodies have been implicated in heart transplantation rejection. However, targets of non-HLA antibodies remain elusive. Here, we utilized a panel of multiplex beads-based assay to determine the specificity of non-HLA antibodies following heart transplantation. We utilized a selected cohort of recipients who did not have HLA donor specific antibodies, but were diagnosed with antibody mediated rejection and treated for antibody mediated rejection. We found the presence of vimentin antibody was associated with treated antibody mediated rejection. Our results suggest that, in heart transplant recipients who are suspected of AMR but in the absence of HLA donor specific antibodies, non-HLA antibodies should be examined.

非人类白细胞抗原 (HLA) 抗体与心脏移植排斥有关。然而，非 HLA 抗体的靶标仍然难以捉摸。在这里，我们利用一组基于多重珠的测定来确定心脏移植后非 HLA 抗体的特异性。我们使用了一组选定的受者，这些受者没有 HLA 供体特异性抗体，但被诊断为抗体介导的排斥反应并接受了抗体介导的排斥反应治疗。我们发现波形蛋白抗体的存在与处理过的抗体介导的排斥有关。我们的结果表明，对于怀疑患有 AMR 但没有 HLA 供体特异性抗体的心脏移植受者，应检查非 HLA 抗体。