Arbuscular mycorrhizal fungi (AMF) plays a key role in enhancing resistance to plant stress and diseases, thus improving soil structure. To better understand the effects of coal mining subsidence and mining-after transplanted trees on soil AMF diversity, this study, using spore isolation as well as identification and high-throughput sequencing techniques, analysed the AMF composition and diversity and its correlation with soil factors from three areas—namely, the original undisturbed area (C), mining-after naturally restoring area (S), and post-subsidence artificially planted 6-year-restoring areas (S1, S2, S3, S4). The results showed significant differences in the AMF diversity of the rhizosphere and non-rhizosphere soil in the coal mining subsidence area. Furthermore, transplanted trees affected the soil TN, AP, and TP nutrients. A total of 15 species from four families and seven genera of AMF were isolated and identified from soil samples in the study areas. The relative abundances of these AMF species were: Glomus (59.83–92.57%), Diversispora (0.59–32.73%), Scutellospora (0.59–7.1%), and others (0–0.05%). The most abundant species were Glomus-lamellosu-VTX00193 (3.65–22.64%), followed by Glomus-viscosum-VTX00063 (1.57–7.58%), Glomus-MO-G18-VTX00064 (0.59–10.93%), and Glomus-perpusillum-VTX00287 (0.62–19.47%). Among them, 13 and 15 species in four families from seven genera were isolated from non-rhizosphere and rhizosphere soil of the transplanted tree species, respectively. The morphological diversity and molecular diversity in C were significantly higher than those in S. Further, the AMF diversity in S3 was higher than that in S. TN, AK, and WC were the most influential soil environmental factors for the AMF community structure in the rhizosphere and non-rhizosphere soil samples of each transplanted tree species. This study found that subsidence exerts unique effects on soil properties, and transplanted trees improved the soil properties and promoted an increase in soil AMF community diversity within the study area, S3 promoted the recovery of morphological and molecular diversity. S2 showed increased species diversity and abundance of dominant species. Therefore, the transplanted shrub mixed forests and Hippophae rhamnoides forests exhibited the most significant effects.

丛枝菌根真菌（AMF）在增强植物抗逆性和抗病性，从而改善土壤结构方面起着关键作用。为了更好地了解采煤沉陷和采伐后移植树木对土壤AMF多样性的影响，本研究使用孢子分离以及鉴定和高通量测序技术，分析了AMF的组成和多样性及其与土壤因子的相关性。这三个区域分别是原始无扰动区（C），自然恢复后采挖区（S）和采后人工种植的6年恢复区（S1，S2，S3，S4）。结果表明，采煤塌陷区根际土壤和非根际土壤的AMF多样性存在显着差异。此外，移植的树木影响了土壤的TN，AP和TP养分。从研究区域的土壤样品中分离并鉴定了来自四个科和七个属的AMF的总共15个物种。这些AMF种类的相对丰度为：球囊（59.83-92.57％），Diversispora呈现（0.59-32.73％），Scutellospora（0.59-7.1％），其他（0-0.05％）。最丰富的物种是Glomus-lamellosu-VTX00193（3.65–22.64％），其次是Glomus-viscosum-VTX00063（1.57–7.58％），Glomus-MO-G18-VTX00064（0.59–10.93％）和Glomus-perpusillum- VTX00287（0.62-19.47％）。其中，分别从移植树种的非根际和根际土壤中分离了七个属的四个科的13种和15种。C中的形态多样性和分子多样性显着高于S。S3中的AMF多样性高于S中的TN。AK和WC是影响AMF群落结构的最土壤环境因素。每种移植树种的根际和非根际土壤样品。本研究发现沉陷对土壤性质具有独特的影响，移植的树木改善了土壤性质并促进了研究区内土壤AMF群落多样性的增加，S3促进了形态和分子多样性的恢复。S2显示出物种多样性的增加和优势物种的丰富。因此，灌木丛混交林和沙棘林表现出最显着的效果。