Enhanced xylanase and endoglucanase production from Beauveria bassiana SAN01, an entomopathogenic fungal endophyte,Fungal Biology

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Enhanced xylanase and endoglucanase production from Beauveria bassiana SAN01, an entomopathogenic fungal endophyte
Fungal Biology ( IF 2.9 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.funbio.2020.10.003
Ayodeji Amobonye ₁ , Prashant Bhagwat ₁ , Suren Singh ₁ , Santhosh Pillai ₁

Affiliation

This study was undertaken to explore alternative applications of the widely known entomopathogenic/endophytic fungus, Beauveria bassiana, besides its sole use as a biocontrol agent. B. bassiana SAN01, was investigated for the production of two glycoside hydrolases, xylanase and endoglucanase under submerged conditions. Among the different biomass tested, wheat bran provided the best results for both xylanase and endoglucanase, and their production levels were further enhanced using response surface methodology. Under optimised conditions, heightened yields of 1061 U/ml and 23.03 U/ml were observed for xylanase and endoglucanase, respectively, which were 3.44 and 1.35 folds higher than their initial yields. These are the highest ever production levels reported for xylanase and endoglucanase from any B. bassiana strain or any known entomopathogenic fungi. Furthermore, the efficacy of xylanase/endoglucanase cocktail in the saccharification of sugarcane bagasse was evaluated. The highest amount of reducing sugar released from the pretreated biomass by the action of the crude Beauveria enzyme cocktail was recorded at 30°C after 8 h incubation. The significant activities of the hydrolytic enzymes recorded with B. bassiana in this study thus present promising avenues for the use of the entomopathogen as a new source of industrial enzymes and by extension, other biotechnological applications.

中文翻译：

增强白僵菌 SAN01（一种昆虫病原真菌内生菌）的木聚糖酶和内切葡聚糖酶的产生

本研究旨在探索广为人知的昆虫病原/内生真菌白僵菌除了作为生物防治剂的唯一用途之外的其他应用。研究了 B. bassiana SAN01 在浸没条件下生产两种糖苷水解酶、木聚糖酶和内切葡聚糖酶。在测试的不同生物质中，麦麸的木聚糖酶和内切葡聚糖酶的结果最好，并且使用响应面方法进一步提高了它们的生产水平。在优化条件下，观察到木聚糖酶和内切葡聚糖酶的产量分别提高了 1061 U/ml 和 23.03 U/ml，比它们的初始产量高 3.44 和 1.35 倍。这些是任何 B. 球孢子菌菌株或任何已知的昆虫病原真菌。此外，还评估了木聚糖酶/内切葡聚糖酶混合物在甘蔗渣糖化中的功效。在 30°C 温育 8 小时后，记录了通过粗白僵菌酶混合物的作用从预处理的生物质中释放的最高量的还原糖。在本研究中用 B. bassiana 记录的水解酶的显着活性因此为使用昆虫病原体作为工业酶的新来源以及其他生物技术应用提供了有希望的途径。在 30°C 温育 8 小时后，记录了通过粗白僵菌酶混合物的作用从预处理的生物质中释放的最高量的还原糖。在本研究中用 B. bassiana 记录的水解酶的显着活性因此为使用昆虫病原体作为工业酶的新来源以及其他生物技术应用提供了有希望的途径。在 30°C 温育 8 小时后，记录了通过粗白僵菌酶混合物的作用从预处理的生物质中释放的最高量的还原糖。在本研究中用 B. bassiana 记录的水解酶的显着活性因此为使用昆虫病原体作为工业酶的新来源以及其他生物技术应用提供了有希望的途径。

更新日期：2021-01-01

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