Essential oil (EO) from the peels of Citrus deliciosa fruits are underexploited among Citrus EO by the food and cosmetic industries. The aims of this study were to evaluate the biological effects of EO from C. deliciosa fruit peels at different stages of maturation in an adenocarcinoma cell line and propose the use of this by-product as a new source for the extraction of bioactive compounds. The fruits were collected at the immature (IM), intermediate (INT), and mature (MAT) stages. A total of 15, 17, and 14 components were measured using GC-MS in the EO at the IM, INT, and MAT stages, respectively. The main components were limonene and γ-terpinene at all the stages. The in vitro antioxidant activity was evaluated using ABTS^+• assay, and EO INT showed the greatest activity with an IC₅₀ value of 0.264 ± 0.004 mg/mL. The cytotoxic effect was evaluated against HT-29 cells, in which EO MAT showed significant cytotoxic activity with an IC₅₀ of 110 ± 10 μg/mL. It was found that the presence of citronellol and β-linalool favors the antioxidant activity; however, their presence suggested a decrease in the cytotoxic activity, when associating the phytochemical profile with the evaluated activities. The results showed that secondary compounds at trace concentrations were associated with differences in biological activities. C. deliciosa fruit peel, a by-product of food juice industry, might be used as a source for the extraction of bioactive compounds with promising biological activities.

食品和化妆品工业在柑橘EO中未充分利用柑桔类水果的果皮中的精油（EO）。这项研究的目的是评估腺癌细胞系中成熟阶段不同阶段来自美味梭状芽胞杆菌果皮的EO的生物学效应，并提出将该副产物用作提取生物活性化合物的新来源。在未成熟（IM），中间（INT）和成熟（MAT）阶段收集果实。使用GC-MS在EO的IM，INT和MAT阶段分别测量了总共15、17和14个组分。在所有阶段，主要成分为柠檬烯和γ-萜品烯。在体外使用ABTS抗氧化活性进行评价^+•分析，EO INT表现出最大的活性，IC ₅₀值为0.264±0.004 mg / mL。评估了对HT-29细胞的细胞毒性作用，其中EO MAT显示出显着的细胞毒性活性，IC ₅₀为110±10μg/ mL。发现香茅醇和β-芳樟醇的存在有利于抗氧化活性。然而，当将植物化学特征与所评估的活性相关联时，它们的存在提示细胞毒性活性降低。结果表明，痕量浓度的次要化合物与生物活性的差异有关。念珠菌 食品果汁工业的副产品果皮可用作提取具有良好生物活性的生物活性化合物的原料。