The increased international trade and worldwide consumption of seafood has increased the possibility of economic fraud. Particularly, the increased development of processed seafood has restricted the application of classical identification methods. DNA matabarcoding is a rapid method for identification of multiple species from an environmental sample. In this study, two universal markers were designed in the mitochondrial cytochrome oxidase subunit I region, and the metabarcoding analysis of processed seafood products was done using these markers. A total of 11 seafood products were analyzed, and 56 and 32 species within 36 families between them were identified by each primer set, respectively. Comparing the identified species with each primer set, only 12.8% of the species were identified by both primer sets. In addition, the species on the label when identified were not observed in some products. Although the analysis was done on the same gene region, different results can be obtained according to the primer set. Based on these results, it is advantageous to use more than one primer set to identify various species from seafood products using DNA metabarcoding analysis.

国际贸易的增加和世界范围内海产品的消费增加了经济欺诈的可能性。特别地，加工海产品的增加发展限制了经典识别方法的应用。DNA matabarcoding是一种从环境样品中鉴定多种物种的快速方法。在这项研究中，在线粒体细胞色素氧化酶亚基I区域设计了两个通用标记，并使用这些标记进行了海产品加工的元条形码分析。总共对11种海鲜产品进行了分析，并通过每个引物对分别鉴定了它们之间36个科中的56种和32种。将鉴定出的物种与每个引物组进行比较，两个引物组仅鉴定出了12.8％的物种。此外，在某些产品中未观察到标签上标明的物种。尽管分析是在相同的基因区域进行的，但根据引物组可以获得不同的结果。基于这些结果，使用多于一种的引物组以利用DNA元条形码分析从海鲜产品中鉴定各种物种是有利的。