Interferon (IFN)γ is a pivotal cytokine that promotes and orchestrates innate cellular and adaptive cell-mediated immunity against intracellular pathogens. The capacity of T cells in mammals to produce IFNγ has been measured using specific antibodies in order to analyze cell-mediated immune responses against infection or immuno-stimulants. In fish, however, measurement of IFNγ protein levels has not been possible due to a lack of research tools. In the present study, therefore, we established antibodies that react with endogenous amberjack IFNγ. An enzyme-linked immunosorbent assay (ELISA) for IFNγ in amberjack species was developed using these antibodies. The ELISA could detect endogenous IFNγ at concentrations less than 100 pg/mL in PMA/ionomycin-stimulated leukocytes culture supernatant. IFNγ production was enhanced and lasted a long time following intracellular bacterial infection with Nocardia seriolae, which is thought to be targeted by cell-mediated immunity. These results demonstrate that quantification of IFNγ using the reported ELISA can be used to estimate the status of cell-mediated immunity in amberjack species.

干扰素（IFN）γ是关键的细胞因子，可促进和协调针对细胞内病原体的先天细胞和适应性细胞介导的免疫力。为了分析细胞介导的针对感染或免疫刺激的免疫反应，已使用特异性抗体测量了哺乳动物T细胞产生IFNγ的能力。但是，在鱼类中，由于缺乏研究工具，无法测量IFNγ蛋白水平。因此，在本研究中，我们建立了与内源性琥珀mberIFNγ反应的抗体。使用这些抗体开发了一种针对琥珀鱼中IFNγ的酶联免疫吸附测定（ELISA）。ELISA可以检测PMA /离子霉素刺激的白细胞培养上清液中浓度低于100 pg / mL的内源性IFNγ。sinoolae诺卡氏菌，被认为是细胞介导的免疫的靶标。这些结果表明，使用报道的ELISA对IFNγ进行定量可用于估计琥珀鱼中细胞介导的免疫状态。