This study sought to characterize sirtuin 2 (sirt2), sirtuin 3 (sirt3), and sirtuin 5 (sirt5) in Megalobrama amblycephala (M. amblycephala) by cloning the open reading frame (ORF) of sirt2, sirt3, and sirt5. The full-lengths of the resulting M. amblycephala sirt2, sirt3, and sirt5 cDNA sequences were 1845, 1534, and 1920 bp, respectively, with 92%, 98%, and 91% similarities to Danio rerio sequences. Based on our bioinformatic analyses and predictions, the sirt2 and sirt3 genes of M. amblycephala were classified within the Sir2 I family, whereas sirt5 belonged to the Sir2 III family. Furthermore, sirt2, sirt3, and sirt5 were widely distributed in different M. amblycephala tissues. Particularly, sirt2 and sirt5 were highly expressed in gills, intestines, and liver (P < 0.05), whereas sirt3 was highly expressed in gills, kidney, liver, and spleen (P < 0.05). A 2 × 2 factorial experiment was also conducted to analyze sirt2, sirt3, and sirt5 expression patterns in response to acute temperature (25 and 32 °C) and ammonia nitrogen (0 and 20 mg/L) stress. Notably, these two stressors were found to interactively affect sirt2, sirt3, and sirt5 expression patterns in M. amblycephala liver. At the higher water temperature (32 °C) and ammonia nitrogen concentration (20 mg/L) tested herein, sirt2, sirt3, and sirt5 had similar expression levels and exhibited a down-regulation trend at 6 and 48 h post-stress but became up-regulated thereafter to counteract the stressors at 96 h post-stress.

本研究旨在表征沉默调节蛋白2（SIRT2），沉默调节蛋白3（SIRT3），和沉默调节蛋白5（SIRT5）在团头鲂（M.鲂）通过克隆SIRT2，SIRT3，和SIRT5的开放阅读框（ORF）。将所得的全长度M.鲂SIRT2，SIRT3，和SIRT5的cDNA序列为1845，1534和1920碱基对，分别用92％，98％，和91间％的相似性斑马鱼的序列。根据我们的生物信息学分析和预测，对SIRT2和SIRT3基因，团头鲂进行了分类Sir2的我的家庭中，而SIRT5属于Sir2的III系列。此外，sirt2，sirt3和sirt5广泛分布在不同的弱视分枝杆菌中。组织。特别是，sirt2和sirt5在腮，肠和肝脏中高表达（P  <0.05），而sirt3在腮，肾脏，肝和脾中高表达（P  <0.05）。还进行了2×2阶乘实验来分析sirt2，sirt3和sirt5在急性温度（25和32°C）和氨氮（0和20 mg / L）胁迫下的表达模式。值得注意的是，发现这两个压力因子相互作用地影响了弱脑杖中的sirt2，sirt3和sirt5表达模式。肝。在本文测试的较高水温（32°C）和氨氮浓度（20 mg / L）下，sirt2，sirt3和sirt5具有相似的表达水平，并在胁迫后6和48 h呈现下调趋势，但变为此后上调以抵消应激后96小时的应激源。