Animal fat is a residue generated from meat processing industries. In the current work, animal fat has been used as carbon source for producing rhamnolipids by Pseudomonas aeruginosa D1. The synthesized rhamnolipids were used to intensify phytoremediation of heavy metal-contaminated soils. First, animal fat was degraded by alkaline and enzymatic hydrolysis methods. Experimental results indicated that the animal fat hydrolysate of alkaline hydrolysis could increase the rhamnolipids yield with a high proportion of mono-rhamnolipids. For achieving the continuous production and separation of rhamnolipids, a coupling strategy of foam fractionation and fermentation with feeding carbon source was developed. Under the conditions of feed flow rate of animal fat hydrolysate 2.0 mL/min, gas volumetric flow rate 90 mL/min and inoculation scale 6.0 %, the yield and recovery percentage of rhamnolipids were 25.8 g/L and 93.4 %, respectively. The synthesized rhamnolipids could significantly improve the activity of soil enzymes for facilitating the digestion and transformation of heavy metals from soils toward the above-ground part of ryegrass.

动物脂肪是从肉类加工工业中产生的残留物。在目前的工作中，动物脂肪已被用作铜绿假单胞菌生产鼠李糖脂的碳源。D1 合成的鼠李糖脂用于加强对重金属污染土壤的植物修复。首先，动物脂肪通过碱性和酶水解方法降解。实验结果表明，碱水解的动物脂肪水解物能以较高的单鼠李糖脂比例提高鼠李糖脂的产量。为了实现鼠李糖脂的连续生产和分离，开发了泡沫分离和发酵与进料碳源的耦合策略。在动物脂肪水解物进料流速为2.0mL / min，气体体积流速为90mL / min，接种规模为6.0％的条件下，鼠李糖脂的得率和回收率分别为25.8g / L和93.4％。