Delayed entry of patient blood culture samples into a microbial detection system is unavoidable at times, due to off-shift staffing or transporting samples to centralized laboratories. Pre-incubation time and temperature of blood culture bottles are the most critical factors impacting recovery and detection of microorganisms. A total of 1377 BACT/ALERT® (BTA) Fastidious Antimicrobial Neutralization (FAN® PLUS) bottles (FA PLUS, FN PLUS, and PF PLUS) were tested after delayed entry times of 24 and 36 h at 20–25 °C (room temperature, RT) prior to loading into the BACT/ALERT® VIRTUO® microbial detection system (VIRTUO). Clinically relevant organisms were inoculated into bottles with 5–84 colony forming units (CFU) per bottle, and human blood (0 to 10 mL), and then loaded into the VIRTUO. When bottles were loaded without delay, a mean time to detection (TTD) of 9.6 h was observed. For delayed bottles, the TTD reported by the VIRTUO was added to the 24-h and 36-h delay times and resulted in average time to results of 32.5 h and 42.5 h, respectively. The FAN PLUS bottles in conjunction with the VIRTUO produced acceptable results when delays up to 24 h at 20–25 °C occur in loading.

由于工作人员下班或将样本运送到中央实验室，有时不可避免地会导致患者血液培养样本延迟进入微生物检测系统。血培养瓶的预培养时间和温度是影响微生物回收和检测的最关键因素。在 20–25 °C（房间温度，RT)，然后装入 BACT/ALERT® VIRTUO® 微生物检测系统 (VIRTUO)。将临床相关微生物接种到每瓶 5-84 菌落形成单位 (CFU) 的瓶子和人血 (0 到 10 mL) 中，然后装入 VIRTUO。当瓶子被毫不拖延地装载时，观察到平均检测时间 (TTD) 为 9.6 小时。对于延迟的瓶子，VIRTUO 报告的 TTD 被添加到 24 小时和 36 小时的延迟时间，分别导致获得结果的平均时间为 32.5 小时和 42.5 小时。FAN PLUS 瓶与 VIRTUO 结合使用时，在 20–25 °C 下加载延迟达 24 小时时，产生了可接受的结果。