Abstract

Nicotiana tabacum is a plant containing cellular T-DNA (T-DNA obtained by plants from Agrobacterium during evolution as a result of horizontal gene transfer) in the genome. Within the species N. tabacum, many varieties are distinguished, and phylogenetic relationships among them have not been established yet. Despite the diversity of phenotypes, N. tabacum varieties are known to have a low level of genetic diversity, so clarification of intraspecific phylogeny is complicated. This article proposes a new marker for studying the phylogeny of the Nicotiana tabacum species on the basis of the results of a comparative analysis of the cT-DNA TA and TB fragments and the PMT2 gene in 11 varieties of Nicotiana tabacum. It has been shown that, for sequences of agrobacterial origin in the genomes of plants, the same evolutionary mechanisms are typical, as for plant sequences. Thus, the cT-DNA (namely, TA) sequences can be used as a phylogenetic marker for the species N. tabacum, since the varieties can be separated into two groups based on the structure of the TA central region.

中文翻译：

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烟草品种农杆菌起源序列的多态性

摘要

烟草是一种在基因组中含有细胞T-DNA（在进化过程中由于水平基因转移而从农杆菌中获得的T-DNA）的植物。在烟草种中，有许多变种，并且它们之间的系统发育关系尚未建立。尽管表型具有多样性，但已知烟草品种的遗传多样性水平很低，因此澄清种内系统发育很复杂。根据对11个烟草品种的cT-DNA TA和TB片段和PMT2基因的比较分析结果，提出了一种用于研究烟草种的系统发育的新标记。烟草。已经表明，对于植物基因组中农杆菌起源的序列，与植物序列相同的进化机制是典型的。因此，cT-DNA（即TA）序列可以用作烟草种的系统发育标记，因为可以根据TA中心区域的结构将其分为两组。