Abstract

A study of the genotoxic activity of fungicide carbendazim was carried out. It was demonstrated that the tested technical grade active ingredient of carbendazim in the dose range of 125–2000 mg/kg body weight induced a statistically significant dose-dependent increase in the frequency of micronucleated polychromatic (PCE) and normochromatic erythrocytes (NCE) in the bone marrow of CD-1 mice. After administration of carbendazim at doses of 250–2000 mg/kg body weight, the accumulation of cells containing two or more micronuclei was observed. According to the results of comet assay, there was no increase in the level of breaks and alkali-labile sites in the DNA of the mouse bone marrow and liver cells after administration of technical grade carbendazim at doses up to 2000 mg/kg body weight. It is therefore suggested that the observed accumulation of cells with micronuclei is due to the aneugenic effect of carbendazim. In addition, a dose-dependent increase in the proportion of macrocytes/gigantocytes and dumbbell-shaped cells with constrictions among PCEs and NCEs was observed, which might be indicative of the inhibitory effect of the test pesticide on cytokinesis. The accumulation of polychromatophiles with pyknotic nuclei after exposure to carbendazim is probably caused by the distortion of nuclear extrusion. A possible general mechanism underlying the impairment of karyokinesis, cytokinesis, and nuclear extrusion in mammalian bone marrow erythroid cells is discussed.

中文翻译：

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多菌灵对小鼠骨髓细胞的细胞遗传学作用

摘要

对杀菌剂多菌灵的遗传毒性进行了研究。结果表明，经测试的多菌灵的工业级活性成分的剂量范围为125-2000 mg / kg体重，导致多巴胺中微核多色（PCE）和正常色红细胞（NCE）的频率具有统计学意义的剂量依赖性增加。 CD-1小鼠的骨髓。多菌灵以250–2000 mg / kg体重的剂量给药后，观察到含有两个或多个微核的细胞蓄积。根据彗星分析的结果，以最高2000 mg / kg体重的剂量施用工业级多菌灵后，小鼠骨髓和肝细胞DNA的断裂水平和碱不稳定位点没有增加。因此，建议观察到的具有微核的细胞积聚是由于多菌灵的气生作用。另外，观察到PCE和NCE之间的大细胞/巨噬细胞和哑铃形细胞比例呈剂量依赖性增加，这可能表明受试农药对胞质分裂的抑制作用。暴露于多菌灵后，具有致密性核的多嗜色细胞的积累可能是由于核挤压变形引起的。讨论了可能的一般机制，其在哺乳动物骨髓红系细胞中损害了核运动，胞质分裂和核挤压。观察到PCE和NCE之间的大细胞/巨噬细胞和哑铃形细胞比例出现剂量依赖性增加，这可能表明受试农药对胞质分裂的抑制作用。暴露于多菌灵后，具有致密性核的多嗜色细胞的积累可能是由于核挤压变形引起的。讨论了可能的一般机制，其在哺乳动物骨髓红系细胞中损害了核运动，胞质分裂和核挤压。观察到PCE和NCE之间收缩的大细胞/巨噬细胞和哑铃形细胞比例呈剂量依赖性增加，这可能表明受试农药对胞质分裂的抑制作用。暴露于多菌灵后，具有致密性核的多嗜色细胞的积累可能是由于核挤压变形引起的。讨论了可能的一般机制，其在哺乳动物骨髓红系细胞中损害了核运动，胞质分裂和核挤压。