Abstract

The double sex and mab-3-related transcription factor 3 gene (DMRT3) placed on chromosome 23 of equine. This gene codes a major transcription factor in the spinal cord complex encompasses regulating motion of vertebrates. It is implicated in a neural network for the synchrony of the moving apparatus that governs limb motion. The aim of this study was to determine polymorphism of DMRT3 gene in Iranian exotic and native horse breeds (Persian Arab, Akhal teke, Yamut, Kurd, Dareshouri, Caspian, Taleshi) using of PCR-RFLP technique and its correlation with body measurements. DNA was extracted from blood samples of 374 horses and a 485-bp fragment within the horse DMRT3 gene was synthesized applying specific primers. The PCR products were digested with DdeI restriction enzyme at 37°C overnight, and the resulting products were separated by the 3.5% agarose gel and visualized by ethidium bromide staining. Body measurements and gene association analyses were performed using a statistical model containing the fixed effects of breed, sex and the animal genotype for the DMRT3 locus. In all of the studied populations two genotypes, CC and AC, were observed while AA was not observed. Frequencies of C and A alleles were 0.97 and 0.03%, respectively. The AC genotype was only observed in Iranian native breeds including Turkmen (Akhal teke), Turkmen (Yamut), Dareshouri, Kurd and Caspian, while the other 12 remained breeds had only CC genotype. Number of observed alleles, number of effective alleles, observed heterozygosity, expected heterozygosity, Shanon’s index, Nei’s index and fixation index (F_is) for all studied breeds were 2.00, 1.06, 0.06, 0.06, 0.14, 0.06 and 0.10, respectively. A UPGMA dendrogram based on the Nei’s standard genetic interval between studied breeds displayed distinct clusters for Iranian native and exotic breeds. Statistical association analysis of two observed genotypes with body measurements showed that the studied mutation has no effect on body measurements.

中文翻译：

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DMRT3基因多态性及其与马品种体测的关系

摘要

位于马的23号染色体上的具有双性恋和mab-3相关转录因子3基因（DMRT3）。该基因编码脊髓复合物中的主要转录因子，涵盖调节脊椎动物的运动。它与神经网络有关，用于控制肢体运动的移动设备的同步性。这项研究的目的是使用PCR-RFLP技术确定DMRT3基因在伊朗外来和本地马种（波斯阿拉伯，阿克哈特克，雅穆特，库尔德，达累斯胡里，​​里海，塔勒西）中的多态性及其与人体测量的相关性。从374匹马的血液样本中提取DNA，并使用特异性引物合成DMRT3基因内的485 bp片段。PCR产物用Dde I限制酶在37°C下过夜，然后将所得产物用3.5％琼脂糖凝胶分离，并通过溴化乙锭染色进行观察。使用统计模型进行身体测量和基因关联分析，该模型包含DMRT3的品种，性别和动物基因型的固定影响轨迹。在所有研究人群中，观察到两种基因型，CC和AC，而未观察到AA。C和A等位基因的频率分别为0.97和0.03％。仅在土库曼（Akhal teke），土库曼（Yamut），Dareshouri，Kurd和里海等伊朗本土品种中观察到AC基因型，而其余12个仅CC基因型的品种。观察到的等位基因数量，有效等位基因数量，观察到的杂合性，预期的杂合性，Shanon指数，Nei指数和固定指数（F_为），所有研究品种分别为2.00、1.06、0.06、0.06、0.14、0.06和0.10。根据研究品种之间Nei的标准遗传间隔的UPGMA树状图显示了伊朗本地和外来品种的不同簇。对两个观察到的基因型与人体测量值的统计关联分析表明，所研究的突变对人体测量值没有影响。