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Whole genome sequencing and phylogenetic classification of Tunisian SARS-CoV-2 strains from patients of the Military Hospital in Tunis
Virus Genes ( IF 1.9 ) Pub Date : 2020-10-09 , DOI: 10.1007/s11262-020-01795-9
Susann Handrick , Malena Bestehorn-Willmann , Simone Eckstein , Mathias C. Walter , Markus H. Antwerpen , Habiba Naija , Kilian Stoecker , Roman Wölfel , Mohamed Ben Moussa

In the present work, two complete genome sequences of SARS-CoV-2 were obtained from nasal swab samples of Tunisian SARS-CoV-2 PCR-positive patients using nanopore sequencing. The virus genomes of two of the patients examined, a Tunisian soldier returning from a mission in Morocco and a member of another Tunisian family, showed significant differences in analyses of the total genome and single nucleotide polymorphisms (SNPs). Phylogenetic relationships with known SARS-CoV-2 genomes in the African region, some European and Middle Eastern countries and initial epidemiological conclusions indicate that the introduction of SARS-CoV-2 into Tunisia from two independent sources was travel-related.



中文翻译:

突尼斯军事医院患者突尼斯SARS-CoV-2毒株的全基因组测序和系统发育分类

在本工作中,使用纳米孔测序从突尼斯SARS-CoV-2 PCR阳性患者的鼻拭子样本中获得了SARS-CoV-2的两个完整基因组序列。被检查的两名患者的病毒基因组,从摩洛哥的一个特派团返回的突尼斯士兵和另一个突尼斯家庭的成员,在总基因组和单核苷酸多态性(SNP)的分析中显示出显着差异。与非洲地区,一些欧洲和中东国家中已知的SARS-CoV-2基因组的亲缘关系和初步的流行病学结论表明,从两个独立的来源将SARS-CoV-2引入突尼斯与旅行有关。

更新日期:2020-10-11
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