Infectious spleen and kidney necrosis virus (ISKNV), causing serious infectious diseases to marine and freshwater fishes, is the type species of the genus Megalocytivirus, family Iridoviridae. In this study, the transcriptional programs of ISKNV in vitro (MFF-1 cells) and in vivo (spleens from mandarin fish) were investigated using real-time PCR. Transcription of all the putative open reading frames (ORFs) of ISKNV was verified. The temporal expression patterns of ISKNV ORFs in vitro and in vivo, including peak expression times (PETs) and relative maximal expression levels, were determined and compared. The K-means clustering with Spearman rank correlation was generated in heat maps constructed based on ISKNV ORF expression profiles in vivo and in vitro. The current study may provide a global picture of ISKNV infection at the transcription level and help better understand the molecular pathogenic mechanism of megalocytiviruses.

脾脏和肾脏坏死病毒（ISKNV）是大轮回病毒属的典型种类，对海洋和淡水鱼类造成严重的传染病。，家庭Iridoviridae。在这项研究中，使用实时PCR研究了ISKNV在体外（MFF-1细胞）和在体内（fish鱼的脾脏）的转录程序。ISKNV的所有假定的开放阅读框（ORF）的转录都得到了验证。确定并比较了ISKNV ORF在体外和体内的时间表达模式，包括峰值表达时间（PET）和相对最大表达水平。在体内和体外基于ISKNV ORF表达谱构建的热图中，生成具有Spearman等级相关性的K-均值聚类。当前的研究可能在转录水平上提供ISKNV感染的全局图，并有助于更好地了解巨轮病毒的分子致病机理。