Key message

Present review summarizes the current applications of nanobodies in plant science and biotechnology, including plant expression of nanobodies, plant biotechnological applications, nanobody-based immunodetection, and nanobody-mediated resistance against plant pathogens.

Abstract

Nanobodies (Nbs) are variable domains of heavy chain-only antibodies (HCAbs) isolated from camelids. In spite of their single domain structure, nanobodies display many unique features, such as small size, high stability, and cryptic epitopes accessibility, which make them ideal for sophisticated applications in plants and animals. In this review, we summarize the current applications of nanobodies in plant science and biotechnology, focusing on nanobody expression in plants, plant biotechnological applications, determination of plant toxins and pathogens, and nanobody-mediated resistance against plant pathogens. Prospects and challenges of nanobody applications in plants are also discussed.

中文翻译：

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纳米抗体在植物科学和生物技术中的应用

关键信息

本综述总结了纳米抗体在植物科学和生物技术中的当前应用，包括纳米抗体的植物表达、植物生物技术应用、基于纳米抗体的免疫检测和纳米抗体介导的植物病原体抗性。

抽象的

纳米抗体 (Nbs) 是从骆驼科动物中分离出来的仅重链抗体 (HCAbs) 的可变结构域。尽管它们具有单域结构，但纳米抗体显示出许多独特的特征，例如小尺寸、高稳定性和神秘的表位可及性，这使其成为植物和动物中复杂应用的理想选择。在这篇综述中，我们总结了纳米抗体在植物科学和生物技术中的当前应用，重点关注纳米抗体在植物中的表达、植物生物技术应用、植物毒素和病原体的测定以及纳米抗体介导的植物病原体抗性。还讨论了纳米体在植物中的应用前景和挑战。