The putative transgenic mungbean (Vigna radiata Var. OBGG-52) plants were regenerated on Murashige and Skoog (1962) medium supplemented with 1.0 mg/L 6-benzylaminopurine (BA). The transgenic status of transformants (T2 generation) was determined through PCR analysis using nptII and ICE1 gene-specific primers as well as uidA reporter gene expression. About 75% of the transformed plantlets were successfully established in the greenhouse. Overexpression of ICE1 gene in transgenic mungbean plants resulted in cold-tolerance at the seedling stage when compared to non-transformed plants. Seeds from T2 generation were further studied for cold tolerance through nutrient culture. Transgenic seeds had significantly increased germination and growth on a nutrient medium at 10–14 °C in growth chamber compared to the non-transgenic control. Moreover, the root and shoot length of transformed plants were significantly increased at 10–14 °C as compared with the non-transformed plants. Under cold stress, it was also observed that the transformed plantlets have significantly higher chlorophyll, proline, and lipid content as well as anti oxidative enzyme activity as compared to non-transformed plants. This study will help in mungbean improvement program for abiotic stress tolerance.

在补充有1.0 mg / L 6-苄基氨基嘌呤（BA）的Murashige和Skoog（1962）培养基上再生推定的转基因绿豆（Vigna radiata Var。OBGG-52）植物。通过PCR分析使用nptII和ICE1基因特异性引物以及uidA报告基因表达来确定转化体的转基因状态（T2代）。在温室中成功建立了约75％的转化苗。ICE1的过表达与未转化的植物相比，转基因的绿豆植物中的基因在幼苗期产生耐寒性。通过营养培养进一步研究了来自T2代的种子的耐寒性。与非转基因对照相比，转基因种子在生长室中10–14°C的营养培养基上的发芽和生长显着增加。此外，与未转化植物相比，在10–14°C时，转化植物的根和茎长显着增加。在冷胁迫下，还观察到与未转化的植物相比，转化的植物具有明显更高的叶绿素，脯氨酸和脂质含量以及抗氧化酶活性。这项研究将有助于改善非生物胁迫耐受性的绿豆计划。