Purpose

Copper oxide (CuO) nanoparticles (NPs) have been utilized in several industries including textile, consumer products, medical, automobiles etc. The discharge of industrial effluents in environment increased the probability of CuO NPs contamination in the ecosystem.

Methods

The present investigation used CuO NPs to determine the toxic effect on Lyngbya species, fresh water algae isolated from natural pond, bacterial species Pseudomonas aeruginosa and Staphylococcus aureus and a crustacean species Daphnia magna.

Results

The NPs average diameter and zeta potential was estimated to be 45 ± 3 nm and 29 ± 1.78 mV respectively. The results showed that 0.1 µg/mL CuO NPs showed the growth inhibition of 47 ± 2% on Lyngbya sp. after 5 days of incubation. The CuO NPs also showed toxic effect to bacterial systems such as P. aeruginosa and S. aureus and crustacean system D. magna. Further, there was an increased lipid peroxidation and generation of reactive oxygen species (ROS) in algal cells observed up on NPs exposure. The exposure of NPs suppressed the antioxidant defense system. The amount of glutathione was reduced after the exposure of NPs.

Conclusion

The study suggested the role of ROS in toxicity of algal and bacterial systems. The present study pointed out the potent toxicity of CuO NPs to the organisms present in the aquatic environment.

中文翻译：

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氧化铜纳米颗粒对绿藻、细菌和甲壳类动物系统的细胞毒理学评价

目的

氧化铜 (CuO) 纳米粒子 (NPs) 已被用于纺织、消费品、医疗、汽车等多个行业。工业废水排放到环境中增加了 CuO NPs 污染生态系统的可能性。

方法

本研究使用 CuO NPs 来确定对Lyngbya物种、从天然池塘中分离的淡水藻类、细菌物种铜绿假单胞菌和金黄色葡萄球菌以及甲壳类物种Daphnia magna的毒性作用。

结果

NPs 的平均直径和 zeta 电位估计分别为 45 ± 3 nm 和 29 ± 1.78 mV。结果表明，0.1 µg/mL CuO NPs 对Lyngbya sp 的生长抑制率为 47 ± 2%。孵化5天后。CuO NPs 对细菌系统如铜绿假单胞菌和金黄色葡萄球菌以及甲壳类动物系统D. magna也显示出毒性作用。此外，在 NPs 暴露下观察到的藻类细胞中脂质过氧化和活性氧 (ROS) 的产生增加。NPs 的暴露抑制了抗氧化防御系统。NPs 暴露后谷胱甘肽的量减少。

结论

该研究提出了 ROS 在藻类和细菌系统毒性中的作用。本研究指出了 CuO NPs 对水生环境中存在的生物体的强毒性。