Objective and design

Methyl gallate (MG) is a prevalent polyphenol in the plant kingdom, which may be related to the effects of several medicinal plants. Although it is widely reported that polyphenols have therapeutic effects, there are few studies demonstrating that MG has anti-inflammatory action. This study aimed to investigate the molecular mechanism behind the anti-inflammatory activity of MG and its effect on hyperalgesia.

Methods

Swiss mice were pretreated orally with different doses of MG and subjected to i.pl. injection of zymosan to induce paw edema. RAW264.7 macrophages and BMDMs stimulated with different TLR agonists such as zymosan, LPS, or Pam3CSK₄ were used to investigate the molecular mechanisms of MG

Results

MG inhibits zymosan-induced paw edema and hyperalgesia and modulates molecular pathways crucial for inflammation development. Pretreatment with MG inhibited cytokines production and NF-κB activity by RAW 264.7 cells stimulated with zymosan, Pam3CSK₄ or LPS, but not with PMA. Moreover, pretreatment with MG decreased IκB degradation, nuclear translocation of NF-κBp65, c-jun and c-fos and ERK1/2, p38 and JNK phosphorylation.

Conclusion

Thus, the results of this study demonstrate that MG has a promising anti-inflammatory effect and suggests an explanation of its mechanism of action through the inhibition of NF-κB signaling and the MAPK pathway.

中文翻译：

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没食子酸甲酯通过抑制MAPK和NF-κb信号通路来减轻Toll样受体配体诱导的炎症

目标和设计

没食子酸甲酯（MG）是植物界中一种普遍存在的多酚，可能与几种药用植物的作用有关。尽管广泛报道多酚具有治疗作用，但很少有研究表明MG具有抗炎作用。本研究旨在探讨MG抗炎活性的分子机制及其对痛觉过敏的作用。

方法

用不同剂量的MG对瑞士小鼠进行口服预处理，并进行i.pl。注射酵母聚糖诱导爪水肿。使用不同的TLR激动剂（如酵母聚糖，LPS或Pam3CSK _4）刺激的RAW264.7巨噬细胞和BMDM来研究MG的分子机制

结果

MG抑制酵母聚糖诱导的爪水肿和痛觉过敏，并调节对于炎症发展至关重要的分子途径。MG预处理可抑制受酵母聚糖，Pam3CSK ₄或LPS刺激的RAW 264.7细胞的细胞因子生成和NF-κB活性，而PMA则不。此外，MG预处理可降低IκB降解，NF-κBp65，c-jun和c-fos的核易位以及ERK1 / 2，p38和JNK磷酸化。

结论

因此，这项研究的结果证明了MG具有良好的抗炎作用，并通过抑制NF-κB信号传导和MAPK途径提出了其作用机理的解释。