Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic relationship and diversity of Fusarium species associated with pink ear rot of maize. Three Fusarium species – F. verticillioides, F. temperatum and F. subglutinans sensu stricto were collected from contaminated corn kernels. A collection of 41 randomly selected Fusarium isolates (16 F. verticillioides, 16 F. temperatum and nine F. subglutinans sensu stricto) was screened for polymorphism with thirty decamer primers. Seventeen primers that had generated a high number of polymorphic loci were used for the analysis of genetic diversity. The primers yielded a total of 146 polymorphic loci, an average of 8.59 polymorphic bands per primer. The percentage of polymorphic bands (%P) was 46.1%. The RAPD-based UPGMA analysis demonstrated that although F. temperatum and F. subglutinans s.s. form two distinct groups they are more similar to each other than to F. verticillioides. Fusarium verticillioides was the most heterogenous species (Nei’s genetic diversity 0.180), while the least heterogenous one was F. subglutinans s.s. whose genetic diversity was 0.098. The analysis of the translation elongation factor 1-α and β-tubulin gene sequences as well as the mating type ratio were employed to support the RAPD data.

利用随机扩增的多态性DNA（RAPD）标记来估计与玉米粉红色腐烂相关的镰刀菌种的遗传关系和多样性。三镰刀菌物种- F.轮枝，F. temperatum和F. subglutinans狭义从受污染的玉米粒收集。41个随机选择的一个集合镰刀菌分离株（16  F.轮枝，16  F. temperatum和九个F. subglutinans用三十种decamer引物筛选了sensu stricto）的多态性。使用十七个产生大量多态性位点的引物来分析遗传多样性。引物总共产生了146个多态性位点，每个引物平均有8.59个多态性条带。多态性带的百分比（％P）为46.1％。基于RAPD的UPGMA分析表明，尽管温度链霉菌和亚谷胶镰刀菌形成两个不同的组，但彼此之间的相似性要好于螺旋藻。枯萎镰刀菌是异质性最高的物种（Nei的遗传多样性为0.180），而异质性最少的是F. subglutinans ss其遗传多样性为0.098。分析翻译延伸因子1-α和β-微管蛋白基因序列以及交配类型比，以支持RAPD数据。