New ion interaction chromatographic (IIC) system with RP column and boric acid plus tetra-n-butylammonium hydroxide (TBAOH) mobile phase was investigated. In the system: CPhenylHexyl—10 mM H3BO3/4 mM TBAOH, a large group of anions viz. F−,IO3−, Cl−, ClO2−, BrO3−, NO2−, Br−, NO3−, ClO3−, I−, HPO42−, SO42−, CrO42−, S2O32−, benzoate−, SCN−, ClO4− and phthalate2−, could be isocratically resolved. A study on the effect of temperature revealed that some ions added to ion exchange are also held in the stationary phase by the hydrophobic adsorption. The retention loss for all anions with time was observed. This effect however, was quite slow and good separations could be obtained even after the column stayed a few hundred hours in the mobile phase.

中文翻译：

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对离子相互作用色谱系统的观察：反相色谱柱——H3BO3/TBAOH 流动相和温度的影响

研究了具有反相色谱柱和硼酸加四正丁基氢氧化铵 (TBAOH) 流动相的新型离子相互作用色谱 (IIC) 系统。在系统中：CPhenylHexyl-10 mM H3BO3/4 mM TBAOH，一大群阴离子，即。F-,IO3-, Cl-, ClO2-, BrO3-, NO2-, Br-, NO3-, ClO3-, I-, HPO42-, SO42-, CrO42-, S2O32-, 苯甲酸盐-, SCN-, ClO4-和邻苯二甲酸酯2-，可以等度解决。对温度影响的研究表明，添加到离子交换中的一些离子也通过疏水吸附保留在固定相中。观察到所有阴离子随时间的保留损失。然而，这种效果非常缓慢，即使在色谱柱在流动相中停留数百小时后仍能获得良好的分离效果。