The responses of total nitrogen (TN) and total phosphorus (TP) removal performance and microbial community to 0–1.2 g/L Fe₃O₄@SiO₂ nanoparticles (NPs) in sequencing batch reactors were investigated. Results showed that an appropriate dose of Fe₃O₄@SiO₂ NPs (0.3 g/L) could promote the removal efficiency of TN and TP. High-throughput sequencing results indicated that microbial richness increased, whereas microbial diversity did not vary upon exposure to 0.1–1.2 g/L Fe₃O₄@SiO₂ NPs. The relative abundances of Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria increased from 11.75%, 3.52%, and 6.77%, respectively, at 0 g/L Fe₃O₄@SiO₂ to 27.05%, 7.21%, and 14.77%, respectively, upon exposure to 0.3 g/L Fe₃O₄@SiO₂. At the genus level, 0.3 g/L Fe₃O₄@SiO₂ NPs enriched norank_f_Nitrosomonadaceae, norank_f_Xanthomonadaceae, Amaricoccus, and Shinella. Real-time quantitative polymerase chain reaction results suggested that the gene copy number of ammonium-oxidizing, nitrite-oxidizing, and denitrifying bacteria population remarkably increased, whereas the number of phosphorus-accumulating organisms slightly increased under long-term exposure to 0.3 g/L Fe₃O₄@SiO₂ NPs. Energy-dispersive spectrum analysis showed that the phosphorus content was higher at 0.3 g/L Fe₃O₄@SiO₂ than at 0 g/L Fe₃O₄@SiO₂. Nitrogen removal primarily occurred through a biological mechanism, while most phosphorus in wastewater may be removed by the combination of physicochemical and biological methods.

研究了分批反应器中总氮（TN）和总磷（TP）的去除性能以及微生物群落对0-1.2 g / L Fe ₃ O ₄ @SiO ₂纳米颗粒（NPs）的响应。结果表明，适当剂量的Fe ₃ O ₄ @SiO ₂ NPs（0.3 g / L）可以提高TN和TP的去除率。高通量测序结果表明，微生物富集度增加，而暴露于0.1–1.2 g / L Fe ₃ O ₄ @SiO ₂ NPs时，微生物多样性没有变化。的相对丰度α-变形菌，β-变形菌，和在暴露于0.3 g / L Fe ₃ O的情况下，γgproteobacteria从0 g / L Fe ₃ O ₄ @SiO ₂分别从11.75％，3.52％和6.77％增加到27.05％，7.21％和14.77％。₄ @SiO ₂。在属水平上，0.3 g / L Fe ₃ O ₄ @SiO ₂ NPs富集了Nomon _ f _亚硝菌科，Norank _ f _ Xanthomonadaceae，Amaricoccus和Shinella。实时定量聚合酶链反应结果表明，在长期暴露于0.3 g / L的条件下，氧化铵，亚硝酸盐氧化和反硝化细菌的基因拷贝数显着增加，而磷积累生物的数量略有增加。 Fe ₃ O ₄ @SiO ₂ NPs。能量色散谱分析表明，磷含量较高以0.3g / L的Fe ₃ ö ₄ @SiO ₂比在0克/升的Fe ₃ ö ₄ @SiO ₂。脱氮主要是通过生物机制发生的，而废水中的大多数磷可以通过物理化学和生物方法相结合的方式去除。