Methicillin-resistant Staphylococcus aureus (MRSA), the most commonly detected drug-resistant microbe in hospitals, adheres to substrates and forms biofilms that are resistant to immunological responses and antimicrobial drugs. Currently, there is a need to develop alternative approaches for treating infections caused by biofilms to prevent delays in wound healing. Silver has long been used as a disinfectant, which is non-specific and has relatively low cytotoxicity. Silver sulfadiazine (SSD) is a chemical complex clinically used for the prevention of wound infections after injury. However, its effects on biofilms are still unclear. In this study, we aimed to analyze the mechanisms underlying SSD action on biofilms formed by MRSA. The antibacterial effects of SSD were a result of silver ions and not sulfadiazine. Ionized silver from SSD in culture media was lower than that from silver nitrate; however, SSD, rather than silver nitrate, eradicated mature biofilms by bacterial killing. In SSD, sulfadiazine selectively bound to biofilms, and silver ions were then liberated. Consequently, the addition of an ion-chelator reduced the bactericidal effects of SSD on biofilms. These results indicate that SSD is an effective compound for the eradication of biofilms; thus, SSD should be used for the removal of biofilms formed on wounds.

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磺胺嘧啶银对耐甲氧西林金黄色葡萄球菌生物膜的影响

耐甲氧西林金黄色葡萄球菌（MRSA）是医院中最常见的耐药菌，它会粘附在基质上并形成对免疫反应和抗菌药物具有抵抗力的生物膜。当前，需要开发用于治疗由生物膜引起的感染以防止伤口愈合延迟的替代方法。银长期以来一直被用作消毒剂，它是非特异性的并且具有相对较低的细胞毒性。磺胺嘧啶银（SSD）是一种化学复合物，临床上用于预防受伤后的伤口感染。但是，其对生物膜的影响仍不清楚。在这项研究中，我们旨在分析SSD对MRSA形成的生物膜起作用的潜在机制。SSD的抗菌作用是银离子而不是磺胺嘧啶的结果。培养基中固态硬盘中的银离子含量低于硝酸银。然而，固态硬盘而不是硝酸银通过细菌杀灭而消除了成熟的生物膜。在SSD中，磺胺嘧啶选择性地结合到生物膜上，然后释放出银离子。因此，添加离子螯合剂减少了SSD对生物膜的杀菌作用。这些结果表明，SSD是根除生物膜的有效化合物。因此，应使用SSD去除伤口上形成的生物膜。这些结果表明，SSD是根除生物膜的有效化合物。因此，应使用SSD去除伤口上形成的生物膜。这些结果表明，SSD是根除生物膜的有效化合物。因此，应使用SSD去除伤口上形成的生物膜。