Understanding the metabolic processes in energy metabolism, particularly during fasted exercise, is a growing area of research. Previous work has focused on measuring metabolites pre and post exercise. This can provide information about the final state of energy metabolism in the participants, but it does not show how these processes vary during the exercise and any subsequent post-exercise period. To address this, the work described here took fasted participants and subjected them to an exercise and rest protocol under laboratory settings, which allowed for breath and blood sampling both pre, during and post exercise. Analysis of the data produced from both the physiological measurements and the untargeted metabolomics measurements showed clear switching between glycolytic and ketolytic metabolism, with the liquid chromatography-mass spectrometry (LC-MS) data showing the separate stages of ketolytic metabolism, notably the transport, release and breakdown of long chain fatty acids. Several signals, putatively identified as short peptides, were observed to change in a pattern similar to that of the ketolytic metabolites. This work highlights the power of untargeted metabolomic methods as an investigative tool for exercise science, both to follow known processes in a more complete way and discover possible novel biomarkers.

中文翻译：

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空旷奔跑：一种代谢组学方法，研究禁食运动和休息期间能量代谢的变化

了解能量代谢中的代谢过程，尤其是在禁食运动中，是一个不断发展的研究领域。先前的工作集中在运动前后的代谢物测量。这可以提供有关参与者体内能量代谢最终状态的信息，但不能显示出这些过程在运动过程中以及随后的运动后过程中如何变化。为了解决这个问题，此处描述的工作使禁食的参与者在实验室环境下进行运动和休息，以允许在运动前，运动中和运动后进行呼吸和血液采样。对通过生理学测量和非靶向代谢组学测量得出的数据的分析表明，糖酵解代谢和酮酵解代谢之间存在明显的切换，液相色谱-质谱（LC-MS）数据显示了酮分解代谢的各个阶段，特别是长链脂肪酸的运输，释放和分解。观察到一些信号（假定鉴定为短肽）以类似于酮分解代谢产物的模式变化。这项工作强调了无针对性的代谢组学方法作为运动科学研究工具的力量，既可以更完整地跟踪已知过程，又可以发现可能的新颖生物标志物。