Active packaging is designed to control the development of decay- and disease-causing microorganisms and is emerging as a promising technology for extending shelf-life, maintaining food safety, reducing waste, and minimizing the risks for foodborne diseases. The goal of this work was to develop and characterize bioactive pullulan-based films, containing rockrose (Cistus ladanifer) essential oil. Among other abundant compounds (camphene, bornyl acetate and trans-pinocarveol), α-pinene was identified as the major compound of rockrose essential oil (39.25%). The essential oil presented stronger antibacterial activity against Gram-positive than against Gram-negative bacteria. The antioxidant results indicate the potential of the developed films to be used to package foods susceptible to oxidation and rancification, thus improving their shelf-life. Also, this study reflects the potential of rockrose essential oil, free or incorporated in pullulan, as a promising quorum sensing inhibitor, since it was able to interrupt intercellular communication, inhibiting violacein production. Electronic microscopy images showed the antibiofilm activity of the films with rockrose essential oil that were able to influence bacterial adhesion, which may be explained by the differences in the surface free energy of the films, as also determined.

中文翻译：

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含岩玫瑰精油的支链淀粉膜在潜在的食品包装中的应用

主动包装旨在控制引起腐烂和疾病的微生物的发展，并且正在成为一种有前途的技术，可以延长保质期，保持食品安全，减少浪费并最大程度地减少食源性疾病的风险。这项工作的目的是开发和表征含有岩蔷薇（Cistus ladanifer）香精油的生物活性支链淀粉基薄膜。在其他丰富的化合物中（，烯，乙酸冰片酯和反式（-pinocarveol），α-pine烯被确定为岩玫瑰精油的主要化合物（39.25％）。精油对革兰氏阳性菌的抗菌作用强于对革兰氏阴性菌的抗菌作用。抗氧化剂的结果表明，已开发的薄膜有潜力用于包装易氧化和酸化的食品，从而提高其保质期。此外，这项研究反映了游离或掺入支链淀粉的岩玫瑰精油作为有希望的群体感应的潜力抑制剂，因为它能够中断细胞间的通讯，抑制紫胶素的产生。电子显微镜图像显示了具有岩玫瑰精油的薄膜的抗生物膜活性，该活性能够影响细菌粘附，这也可以通过薄膜表面自由能的差异来解释。