Abstract

The family Peronosporaceae, an obligate biotrophic group of Oomycota, causes downy mildew disease on many cultivated and ornamental plants such as beet, cucumber, grape, onion, rose, spinach, and sunflower. To investigate the diversity of Peronosporaceae species in Korea, we performed morphological analysis for dried plant herbariums with downy mildew infections by two largest genera, Peronospora and Plasmopara. As a result, it was confirmed that there are five species of Peronospora and two species of Plasmopara, which have been so far unrecorded in Korea, as well as rarely known in the world; Pl. angustiterminalis (ex Xanthium strumarium), Pl. siegesbeckiae (ex Siegesbeckia glabrescens), P. chenopodii-ambrosioidis (ex Chenopodium ambrosioides), P. chenopodii-ficifolii (ex Chenopodium ficifolium), P. clinopodii (ex Clinopodium cf. vulgare), P. elsholtziae (ex Elsholtzia ciliata), and P. lathyrina (ex Lathyrus japonicus). In addition, their phylogenetic relationship was inferred by molecular sequence analysis of ITS, LSU rDNA, and cox2 mtDNA. By rediscovering the seven missing species and barcoding their DNA sequences, this study provides valuable insights into the diversity and evolutionary studies of downy mildew pathogens.

摘要

Peronosporaceae科是Oomycota的专性生物营养组，它对许多栽培和观赏植物如甜菜，黄瓜，葡萄，洋葱，玫瑰，菠菜和向日葵造成霜霉病。为了调查韩国Peronosporaceae物种的多样性，我们对两个最大属Peronospora和Plasmopara进行了霜霉病感染的干燥植物标本室进行了形态分析。结果，证实了迄今为止在韩国尚未记录的，在世界上鲜为人知的Peronospora有5种和Plasmopara有2种。Pl。Angustiterminalis（来自Xanthium strumarium），皮尔。西格氏菌（前豨莶），P. chenopodii-ambrosioidis（例如土荆芥），P. chenopodii-ficifolii（前藜ficifolium），P.断血流（例如风轮参见大麦），P. elsholtziae（前香薷），和P 。lathyrina（原Lathyrus japonicus）。另外，通过ITS，LSU rDNA和cox2的分子序列分析可以推断出它们的系统发育关系。线粒体 通过重新发现七个失踪物种并条形码编码其DNA序列，这项研究为霜霉病病原体的多样性和进化研究提供了宝贵的见解。