ABSTRACT Coronavirus is a virus with an external protective membrane known as pericapsid and composed essentially by a double layer of phospholipids (PPL) and other components. The destabilization or the decomposition of this pericapsid means the certain inactivation of the coronavirus. The purpose of this work is to show that ozone is able to oxidize easily the phospholipids destabilizing the micellar state of phospholipidic model compounds derived from soy lecithin. PPL were ozonized in reversal micelle state in n-hexane and in normal micelle state in aqueous emulsion. The progress of PPL ozonation was followed spectrophotometrically and iodometrically. It was found that in n-hexane PPL ozonides (PPL-O) start to precipitate at O3/PPL = 1.5 molar ratio and the precipitation is complete at a molar ratio = 3, confirming the average presence of three double bonds per PPL molecule. The pseudofirst order kinetics of the reverse micelle destabilization oxidation to PPL-O was determined in the range of 1.98–6.75 × 10−3 s−1. On the other hand, PPL in aqueous medium forms a milky emulsion with the PPL molecules arranged in the micelle. The action of ozone on this emulsion causes the complete destabilization of the micelles due to the oxidation of the unsaturated fatty chains of PPL and at O3/PPL = 3 molar ratio the solution appears completely transparent, i.e. the original micellar state is disappeared at a rate of 3.0 × 10−3 s−1. PPL-O was also studied by FT-IR, DSC and polarimetry. The results derived from the ozonation of PPL model compound may be applied to the PPL double layer of coronavirus pericapsid, confirming the effectiveness of ozone in coronavirus inactivation.

中文翻译：

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关于臭氧对磷脂的作用，磷脂是冠状病毒等衣壳病毒外膜的模型化合物。第1部分

摘要 冠状病毒是一种具有称为衣壳的外部保护膜的病毒，主要由双层磷脂 (PPL) 和其他成分组成。这种衣壳的不稳定或分解意味着冠状病毒的某些灭活。这项工作的目的是表明臭氧能够轻松氧化磷脂，破坏源自大豆卵磷脂的磷脂模型化合物的胶束状态。PPL 在正己烷中以反转胶束状态和在水乳液中以正常胶束状态臭氧化。通过分光光度法和碘量法跟踪 PPL 臭氧化的进展。发现在正己烷中 PPL 臭氧化物 (PPL-O) 在 O3/PPL = 1.5 摩尔比时开始沉淀，在摩尔比 = 3 时沉淀完全，确认每个 PPL 分子平均存在三个双键。反胶束去稳定氧化为 PPL-O 的伪一级动力学在 1.98–6.75 × 10-3 s-1 范围内确定。另一方面，PPL 在水性介质中形成乳状乳液，PPL 分子排列在胶束中。由于 PPL 的不饱和脂肪链的氧化，臭氧对该乳液的作用导致胶束完全不稳定，在 O3/PPL = 3 摩尔比下，溶液看起来完全透明，即原始胶束状态以一定的速度消失3.0 × 10−3 s−1。还通过 FT-IR、DSC 和偏振法研究了 PPL-O。PPL模型化合物臭氧化的结果可能适用于冠状病毒衣壳的PPL双层，