Abstract

Leishmania amazonensis is a species causative of cutaneous and anergic diffuse cutaneous leishmaniasis, treatment-resistant form, in the New World. Plants essential oils exhibit great potential as microbicide agents. We described the composition of the essential oils of two plants native from Brazil, Myrcia ovata, with geranial and neral as major constituents, and Eremanthus erythropappus, with α-bisabolol. In vitro effects of these essential oils on L. amazonensis promastigotes growth and ultrastructure were analysed as well as their cytotoxicity to murine macrophages. Both oils were highly active with IC₅₀/96 h of 8.69 and 9.53 µg/mL for M. ovata and E. erythropappus against promastigotes and caused ultrastructural alterations including mitochondrial enlargement. Cytotoxicity for murine macrophages varied with the oil concentrations. The IC₅₀ low values of both M. ovata and E. erythropappus oils against L. amazonensis and their relative low cytotoxicity to mammal host cells support their potential use against cutaneous leishmaniasis.

摘要

Leishmania amazonensis是一种在新世界引起皮肤和无反应性弥漫性皮肤利什曼病，抗治疗形式的物种。植物精油作为杀菌剂表现出巨大的潜力。我们描述了两种原产于巴西的植物Myrcia ovata 的精油成分，其主要成分是天竺葵和 neral，而Eremanthus erythropappus 则含有 α-没药醇。分析了这些精油对L. amazonensis前鞭毛体生长和超微结构的体外影响，以及它们对小鼠巨噬细胞的细胞毒性。两种油是高度活性与集成电路₅₀的8.69 / 96小时和9.53微克/毫升为M.卵形和E. erythropappus对抗前鞭毛体并引起超微结构改变，包括线粒体增大。小鼠巨噬细胞的细胞毒性随油浓度而变化。M. ovata和E. erythropappus油对L. amazonensis的 IC ₅₀低值以及它们对哺乳动物宿主细胞的相对低的细胞毒性支持它们在对抗皮肤利什曼病方面的潜在用途。