ABSTRACT

Yangjiang bermudagrass is an excellent variety with high turf quality and good stress tolerance; however, the inflorescence density of Yangjiang is too high, which leads to management problems. To lower the inflorescence density of Yangjiang bermudagrass, Yangjiang bermudagrass mutants were generated using gamma-ray irradiation. Forty-two mutants were generated, and identification was performed on the basis of eight morphological characteristics and SRAP molecular markers. The results showed that significant variation occurred for all characteristics among the mutants and their parent. Most of the mutants showed reduced turf height; reduced leaf length, internode length and number of reproductive branches; and reduced leaf width. Compared with Yangjiang, some mutants showed higher turf density. The inflorescence density of all tested samples was observed in June, August and November, and the inflorescence density of some mutants was always lower than that of their Yangjiang parent. Based on the morphological characteristics, 12 mutants with low inflorescence density were selected as promising progeny, and their genetic characteristics were identified by SRAP molecular markers. The results showed that all 12 mutants presented differences in amplified polymorphic fragments compared with those of Yangjiang, indicating that mutations at the DNA level occurred in all mutants.

摘要

阳江百慕大草是优良的品种，草皮质量高，抗逆性好。但是阳江的花序密度过高，导致管理上的困难。为了降低阳江百慕大的花序密度，使用γ射线辐照产生了阳江百慕大突变体。产生了42个突变体，并基于八个形态学特征和SRAP分子标记进行了鉴定。结果表明，突变体及其亲本的所有特征均发生了显着变化。大多数突变体的草皮高度降低。减少叶片长度，节间长度和生殖分支数量；并减少叶宽。与阳江相比，一些突变体的草皮密度更高。在6月，8月和11月观察到所有测试样品的花序密度，并且某些突变体的花序密度总是低于其阳江亲本的。根据形态特征，选择低花序密度的12个突变体为有前途的后代，并通过SRAP分子标记鉴定其遗传特性。结果表明，与阳江相比，所有12个突变体均表现出扩增的多态性片段差异，表明所有突变体均发生了DNA水平的突变。并通过SRAP分子标记鉴定其遗传特性。结果表明，与阳江相比，所有12个突变体均表现出扩增的多态性片段差异，表明所有突变体均发生了DNA水平的突变。通过SRAP分子标记鉴定其遗传特性。结果表明，与阳江相比，所有12个突变体均表现出扩增的多态性片段差异，表明所有突变体均发生了DNA水平的突变。