ABSTRACT

Potato is the most important non-grain food crop in the world. Viruses, particularly potato virus Y (PVY) and potato virus A (PVA), are among the major agricultural pathogens causing severe reduction in potato yield and quality worldwide. Virus infection induces host factors to interfere with its infection cycle. Evaluation of these factors facilitates the development of intrinsic resistance to plant viruses. In this study, a small G-protein as one of the critical signaling factors was evaluated in plant response to PVY and PVA to enhance resistance. For this purpose, the gene expression dataset of G-proteins in potato plant under five biotic (viruses, bacteria, fungi, nematodes, and insects) and four abiotic (cold, heat, salinity, and drought) stress conditions were collected from gene expression databases. We reduced the number of the selected G-proteins to a single protein, StSAR1A, which is possibly involved in virus inhibition. StSAR1A overexpressed transgenic plants were created via the Agrobacterium-mediated method. Real-time PCR and Enzyme-linked immunosorbent assay tests of transgenic plants mechanically inoculated with PVY and PVA indicated that the overexpression of StSAR1A gene enhanced resistance to both viruses. The virus-infected transgenic plants exhibited a greater stem length, a larger leaf size, a higher fresh/dry weight, and a greater node number than those of the wild-type plants. The maximal photochemical efficiency of photosystem II, stomatal conductivity, and net photosynthetic rate in the virus-infected transgenic plants were also obviously higher than those of the control. The present study may help to understand aspects of resistance against viruses.

摘要

马铃薯是世界上最重要的非粮食作物。病毒，特别是马铃薯病毒 Y (PVY) 和马铃薯病毒 A (PVA)，是导致全球马铃薯产量和质量严重下降的主要农业病原体。病毒感染诱导宿主因素干扰其感染周期。对这些因素的评估促进了对植物病毒的内在抗性的发展。在这项研究中，作为关键信号因子之一的小 G 蛋白在植物对 PVY 和 PVA 的反应中进行了评估，以增强抗性。为此，从基因表达中收集了马铃薯植物在五种生物（病毒、细菌、真菌、线虫和昆虫）和四种非生物（冷、热、盐度和干旱）胁迫条件下 G 蛋白的基因表达数据集。数据库。StSAR1A，可能与病毒抑制有关。StSAR1A过表达的转基因植物是通过农杆菌介导的方法创建的。机械接种 PVY 和 PVA 的转基因植物的实时 PCR 和酶联免疫吸附试验表明StSAR1A的过表达基因增强了对这两种病毒的抵抗力。与野生型植物相比，病毒感染的转基因植物表现出更长的茎长、更大的叶片、更高的鲜重/干重和更多的节数。病毒感染的转基因植株的光系统II最大光化学效率、气孔导率和净光合速​​率也明显高于对照。本研究可能有助于了解对病毒的抵抗力的各个方面。