Seed vigor is crucial for crop early establishment in the field and is particularly important for forage crop production. Oat (Avena sativa L.) is a nutritious food crop and also a valuable forage crop. However, little is known about the genetics of seed vigor in oats. To investigate seed vigor-related traits and their genetic architecture in oats, we developed an easy-to-implement image-based phenotyping pipeline and applied it to 650 elite oat lines from the Collaborative Oat Research Enterprise (CORE). Root number, root surface area, and shoot length were measured in two replicates. Variables such as growth rate were derived. Using a genome-wide association (GWA) approach, we identified 34 and 16 unique loci associated with root traits and shoot traits, respectively, which corresponded to 41 and 16 unique SNPs at a false discovery rate < 0.1. Nine root-associated loci were organized into four sets of homeologous regions, while nine shoot-associated loci were organized into three sets of homeologous regions. The context sequences of five trait-associated markers matched to the sequences of rice, Brachypodium and maize (E-value < 10^–10), including three markers matched to known gene models with potential involvement in seed vigor. These were a glucuronosyltransferase, a mitochondrial carrier protein domain containing protein, and an iron-sulfur cluster protein. This study presents the first GWA study on oat seed vigor and data of this study can provide guidelines and foundation for further investigations.

种子活力对于田间作物的早期建立至关重要，对饲料作物的生产尤其重要。燕麦（燕麦）L.）是一种营养食品，也是一种宝贵的饲料作物。但是，关于燕麦种子活力的遗传学知之甚少。为了研究燕麦中与种子活力相关的性状及其遗传结构，我们开发了一种易于实现的基于图像的表型分型管道，并将其应用于来自协作燕麦研究企业（CORE）的650个优质燕麦品系。重复两次测量根数，根表面积和枝条长度。得出诸如增长率之类的变量。使用全基因组关联（GWA）方法，我们分别确定了与根性状和枝条性状相关的34和16个唯一基因座，它们以错误发现率<0.1对应于41和16个唯一SNP。九个与根相关的基因座被组织成四组同源区域，同时将9个与芽相关的基因座组织成3组同源区域。与水稻的序列相匹配的五个性状相关标记的背景序列，短芽孢杆菌和玉米（E值<10 ^-10），包括与已知基因模型匹配的三个标记，可能与种子活力相关。这些是葡糖醛酸糖基转移酶，包含线粒体载体蛋白的蛋白结构域和铁硫簇蛋白。这项研究是GWA首次进行的燕麦种子活力研究，该数据可为进一步研究提供指导和基础。