ABSTRACT: Gastritis and gastric ulcers are well-recognized symptoms in cetaceans, and the genus Helicobacter is considered as the main cause. In this study, we examined the gastric fluid of captive common bottlenose dolphins Tursiops truncatus with gastric diseases in order to isolate the organisms responsible for diagnosis and treatment. Four Gram-negative, rod-shaped isolates (TSB^T, TSH1, TSZ, and TSH3) with tightly coiled spirals with 2-4 turns and 2-6 bipolar, sheathed flagella, were obtained from gastric fluids of common bottlenose dolphins with gastric diseases. Phylogenetic analysis, based on 16S rRNA, atpA, and 60 kDa heat-shock protein (hsp60) genes, demonstrated that these isolates form a novel lineage within the genus Helicobacter. Analyses of 16S rRNA, atpA, and hsp60 gene sequences showed that isolate TSB^T was most closely related to H. cetorum MIT99-5656^T (98.5% similarity), H. pylori ATCC 43504^T (76.7% similarity), and H. pylori ATCC 43504^T (78.0% similarity), respectively. Type strains of Helicobacter showing resistance to 2% NaCl have not been reported previously; however, these novel isolates were resistant to 2% NaCl. Culture supernatant of some isolates induced intracellular vacuolization in mammalian cultured cells. These data, together with the different morphological and biochemical characteristics of the isolates, reveal that these isolates represent a novel species for which we propose the name Helicobacter delphinicola sp. nov. with type strain TSB^T (= JCM 32789^T = TSD-183^T). Future studies will confirm whether H. delphinicola plays a role in lesion etiopathogenesis in cetaceans.

中文翻译：

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幽门螺杆菌 十一月，分离自患有胃病的普通宽吻海豚Tursiops truncatus

摘要：胃炎和胃溃疡是鲸类动物中公认的症状，幽门螺杆菌属被认为是主要原因。在这项研究中，我们检查了圈养的常见宽吻海豚Tursiops truncatus的胃液，以分离出负责诊断和治疗的微生物。从患有胃病的普通宽吻海豚的胃液中获得4个革兰氏阴性，棒状分离株（TSB ^T，TSH1，TSZ和TSH3），螺旋紧密缠绕，螺旋缠绕2-4圈，并带有2-6个双极鞘鞭毛。 。系统发育分析，基于16S rRNA，atp A和60 kDa热激蛋白（hsp60）基因，证明这些分离物在幽门螺杆菌属内形成了新的谱系。对16S rRNA，atp A和hsp 60基因序列的分析表明，分离的TSB ^T与cetorum MIT99-5656 ^T（98.5％相似性），H。pylori ATCC 43504 ^T（76.7％相似性）和H幽门螺旋杆菌ATCC 43504 ^Ť分别（78.0％相似性）。幽门螺杆菌的类型菌株以前没有报道过对2％NaCl的抗性；但是，这些新型分离株对2％NaCl具有抗性。一些分离物的培养上清液在哺乳动物培养细胞中诱导细胞内空泡化。这些数据，以及分离物的不同形态和生化特征，揭示了这些分离物代表了一个新的物种，为此我们建议命名为Helicobacter delphinicola sp。十一月 类型为TSB ^T（= JCM 32789 ^T = TSD-183 ^T）。未来的研究将证实，H。delphinicola是否在鲸类的病变病因发病中起作用。