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Shortening the generation cycle in faba bean (Vicia faba) by application of cytokinin and cold stress to assist speed breeding
Plant Breeding ( IF 1.5 ) Pub Date : 2020-10-07 , DOI: 10.1111/pbr.12868
Saeid Mobini 1 , Hamid Khazaei 1 , Thomas D. Warkentin 1 , Albert Vandenberg 1
Affiliation  

The aim of this study was to reduce the length of the breeding cycle for faba bean by accelerating seed setting. We examined the mode and time of exogenous 6‐benzylaminopurine (BAP) (cytokinin) application, and cold treatments and their combinations in two faba bean genotypes. Acropetal node number of pod and seed set and pollen viability were recorded during the experiments. Application of BAP improved pollen germination. The application of 10–5 M BAP 4 days after flowering increased seed set at the lower nodes. Cold treatment (8/4°C day/night for 2 days) after the onset of flowering induced the formation of more pods and faster pod set compared to the non‐cold treatment. The time to first seed was significantly reduced, and pollen viability was increased in plants exposed to cold treatment. Increased pollen viability also showed a significant positive correlation with seed setting. The combinations of 10–5 BAP and cold treatment together had similar and independent effects. These results will accelerate plant breeding in faba bean by providing additional tools for reducing generation time.

中文翻译:

通过应用细胞分裂素和冷胁迫来缩短蚕豆(Vicia faba)的生成周期,以协助快速育种

这项研究的目的是通过加速结实来缩短蚕豆的育种周期。我们检查了外源6-苄氨基嘌呤(BAP)(细胞分裂素)的应用方式和时间,以及两种蚕豆基因型的冷处理及其组合。在实验过程中记录豆荚和种子结实的顶角节数和花粉生存力。BAP的应用改善了花粉的萌发。10 –5的应用 开花后4天的M BAP使下节的结实增加。与非冷处理相比,开花后的冷处理(8/4°C日/夜,持续2天)诱导了更多豆荚的形成和更快的豆荚凝固。暴露于冷处理的植物中,首次播种的时间显着减少,花粉活力增加。花粉生存力的增加也显示出与结实的显着正相关。10 –5 BAP和冷疗的组合具有相似和独立的作用。这些结果将通过提供减少生成时间的其他工具来加速蚕豆的植物育种。
更新日期:2020-10-07
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