The incidence of colon cancer recurrence and metastasis is known to increase as an adverse effect related to postoperative infection. Lipopolysaccharide or LPS, which is derived from gram‐negative bacteria, is a key inducer of inflammatory‐related tumor metastasis. Although there are numerous known biological effects of purple rice extract (PRE), its protective effect on colon metastasis was unknown. This study first evaluated the effects of hexane soluble fraction (HSF) or γ‐oryzanol‐rich fraction of PRE on LPS‐induced colon cancer adhesion and invasion, which was accomplished using adhesive and invasive assay. Gelatin zymography was also utilized for gelatinase activity and secretion. Its chelating activity was also further analyzed by reverse gelatin zymography with zinc chloride. The study findings support the synergistic effect of HSF in protection against adverse events from LPS‐induced colon cancer metastasis, as shown by effects on adhesive and invasive ability as well as matrix metalloproteinase‐2 secretion and activity.

中文翻译：

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紫米提取物中富含γ-谷维素的馏分对脂多糖诱导的人结肠癌细胞转移的抑制作用

众所周知，结肠癌复发和转移的发生率是与术后感染相关的不良反应。源自革兰氏阴性细菌的脂多糖或LPS是炎症相关肿瘤转移的关键诱导剂。尽管紫米提取物（PRE）有许多已知的生物学作用，但其对结肠转移的保护作用尚不清楚。这项研究首先评估了PRE的可溶于己烷的馏分（HSF）或富含γ-谷维素的馏分对LPS诱导的结肠癌黏附和侵袭的影响，这是通过粘附和侵袭性测定实现的。明胶酶谱也用于明胶酶活性和分泌。还通过用氯化锌的反向明胶酶谱进一步分析了其螯合活性。