Genome editing has powerful applications in research, healthcare, and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on, and away from, the target locus. This has considerable impact in providing a safe approach for therapeutic genome editing, agriculture, and other applications. This opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.

基因组编辑在研究、医疗保健和农业方面有着强大的应用。然而，基因组编辑可能产生的分子事件的范围被低估了，并且该技术在目标基因座上和远离目标基因座仍然是不可预测的。这对于为治疗性基因组编辑、农业和其他应用提供安全方法具有相当大的影响。这篇评论文章讨论了如何通过组合检测来预测和检测这些编辑事件，以捕获所有可能的基因组变化。它还讨论了防止不良影响的策略，这对于评估与使用该技术相关的收益或风险至关重要。预测和验证基因组编辑的结果对于所有应用的成功至关重要。