A new way of electrochemical DNA sensor using as a screening tool for the determination of phytochemicals with high genoprotective functionality is proposed. The biosensor's detection layer was prepared with double stranded deoxyribonucleic acid (ds DNA) that were subjected to oxidative stress induced by ^•OH radicals generated by Fenton reaction. The oxidized guanine derivative, 8-oxo-7,8-dihydro-2′-deoxyguanosine, was treated as an indicator of DNA oxidative damage. This derivative may cause mutation through its ability to pair with adenine. The abnormalities of DNA structure and DNA repair system are known to be directly related to progressive neurodegeneration. The present study showed that during oxidative stress, the 2.5% oregano extract protected guanine from undergoing oxidation to 8-oxoguanine. The results revealed that this genoprotective effectiveness can make oregano a very efficient protective barrier against oxidative stress. Due to these unique properties of oregano we propose the recipe of a functional bread with its addition. It was found that the functionality of the prepared bread was not limited to antioxidative activity but also is expressed in the inhibition of cholinesterases. These findings indicate that oregano can act as an important component in the therapeutic diet recommended in neurodegenerative disorders.

提出了一种新的电化学DNA传感器作为筛选工具，用于测定具有高基因保护功能的植物化学物质。用该进行由诱导的氧化应激的双链脱氧核糖核酸（DNA DS）中制备的生物传感器的检测层^•通过Fenton反应生成的OH自由基。氧化的鸟嘌呤衍生物8-oxo-7,8-dihydro-2'-deoxyguanosine被视为DNA氧化损伤的指标。该衍生物可能通过与腺嘌呤配对的能力引起突变。已知DNA结构和DNA修复系统的异常与进行性神经变性直接相关。本研究表明，在氧化应激期间，2.5％的牛至提取物可保护鸟嘌呤免于氧化为8-氧代鸟嘌呤。结果表明，这种基因保护作用可使牛至成为对抗氧化应激的非常有效的保护屏障。由于牛至的这些独特特性，我们建议添加功能性面包。发现制备的面包的功能性不仅限于抗氧化活性，而且在抑制胆碱酯酶中表达。这些发现表明，牛至可以作为神经退行性疾病推荐的治疗饮食中的重要成分。