CHROMagar Enterococcus (CHR), a new chromogenic medium not yet available for commercial purchase, was evaluated for the isolation of Enterococcus from environmental water samples. Its performance was evaluated in comparison to commercially available media, Enterococcosel agar and m-Enterococcus agar. Three consecutive tests were conducted with each test being performed with a newer batch of the CHR medium with improved media composition per batch. The recovery rate, positive predictive value, and sensitivity of the CHR medium improved with the subsequent re-formulation of the media components from 93.9%, 63%, and 92.6%, respectively, with the first batch of CHR, to 96.2%, 97.4%, and 95.7%, respectively, with the newest batch of CHR. The results showed that the newer batches of CHR performed better than the previous versions and are comparable to the other two commercial media tested. The CHR medium has been developed to decrease the turnaround time to approximately 18 h and be read more easily due to bigger colony morphology. The superior growth of colonies on CHR compared with other media in a shorter period of time can aid in the early detection of enterococci and may offer a user-friendly alternative to other media for the isolation of enterococci.

科玛嘉肠球菌(CHR) 是一种尚未可供商业购买的新型显色培养基，用于从环境水样中分离肠球菌进行评估。其性能通过与市售培养基、肠球菌琼脂和间肠球菌琼脂进行比较来评估。进行了三次连续测试，每次测试均使用每批次改进的培养基成分的新批次 CHR 培养基进行。随着随后重新配制培养基成分，CHR 培养基的回收率、阳性预测值和敏感性分别从第一批 CHR 的 93.9%、63% 和 92.6% 提高到 96.2%、97.4%最新批次的 CHR 分别为 % 和 95.7%。结果表明，新批次的 CHR 性能优于之前的版本，并且与测试的其他两种商业介质相当。 CHR 培养基的开发可将周转时间缩短至约 18 小时，并且由于菌落形态较大，因此更容易读取。与其他培养基相比，CHR 上的菌落在更短的时间内生长良好，有助于肠球菌的早期检测，并可能为分离肠球菌提供一种用户友好的替代其他培养基的方法。