The present study investigated the effect of carbohydrase enzyme, methionine (Met) (100% above Cobb 500 requirement level), or their combination on growth performance, carcass yield, total tract retention (TTR) of DM, CP, fatty acid (FA), muscle FA composition, lipid peroxidation products, and jejunum histomorphology in broiler chickens fed 10% flaxseed-containing diets. One hundred and twenty (n = 120) day-old Cobb chicks were fed corn-soybean meal–based diet containing 0% flaxseed (control), 10% whole flaxseed (diet 1), diet 1 + 0.05% carbohydrase enzyme (diet 2), diet 1 + 100% more Cobb 500 Met requirement (diet 3), and diet 1 + 0.05% carbohydrase enzyme + 100% more Cobb 500 Met requirement (diet 4) for 42 d. The data were analyzed by ANOVA with 5 treatments with 4 replications in a completely randomized design. At day 42, BW gain was the highest in control when compared to flaxseed-containing diets (P < 0.05). Dry matter TTR was higher in diet 3 and diet 4 than that in diet 1 and diet 2 (P < 0.05), and CP TTR was highest in diet 3 compared with that in diet 1 and diet 2 (P < 0.05). Supplementing Met increased linoleic and α-linolenic acid TTR in birds fed flaxseed (P < 0.05). Total and long-chain (≥20C) n-3 FA was higher in the thigh muscle of broilers fed diet 3 and diet 4 than in those fed diet 1, diet 2, and control (P < 0.05). Lipid oxidation products in the thigh muscle was the highest in birds fed diet 4 (P < 0.05). Jejunal villous height, villous perimeter, and villous surface area were highest in birds fed diet 1 (P < 0.05). In conclusion, supplementing Met, or its combination with carbohydrase enzyme, increased DM, CP, and essential FA TTR and thigh muscle n-3 FA in broilers fed flaxseed. However, supplementing Met or its combination reduced BW gain and negatively affected jejunal morphology in broilers fed flaxseed.

本研究调查了碳水化合物酶，蛋氨酸（Met）（高于Cobb 500要求水平的100％）或它们的组合对DM，CP，脂肪酸（FA）的生长性能、,体产量，总道保留率（TTR）的影响），饲喂含10％亚麻籽的肉鸡的肌肉FA组成，脂质过氧化产物和空肠组织形态。向120只（n = 120）日龄的Cobb雏鸡饲喂以玉米豆粕为基础的日粮，该日粮含0％亚麻籽（对照），10％全亚麻籽（饮食1），饮食1 + 0.05％碳水化合物酶（饮食2） ），饮食1 + 100％Cobb 500 Met需求量（饮食3），饮食1 + 0.05％碳水化合物酶+ 100％Cobb 500 Met需求量（饮食4）持续42 d。在完全随机的设计中，通过ANOVA分析5种治疗方法，重复4次，分析数据。与含亚麻籽的日粮相比，在第42天，对照中的体重增加最高（P <0.05）。饮食3和4的干物质TTR高于饮食1和2的干物质TTR（P<0.05），与饮食1和饮食2相比，饮食3中的CP TTR最高（P <0.05）。补充Met可提高亚麻籽饲喂家禽的亚油酸和α-亚麻酸的TTR（P <0.05）。饲喂日粮3和日粮4的肉鸡大腿肌肉的总链和长链（≥20C）n-3 FA高于饲喂日粮1，日粮2和对照的肉鸡的大腿肌肉（P <0.05）。饲喂4日的家禽，大腿肌肉中的脂质氧化产物最高（P <0.05）。饲喂日粮1的空肠绒毛高度，绒毛周长和绒毛表面积最高（P<0.05）。总之，在饲喂亚麻籽肉鸡的肉鸡中，补充Met或与糖酶混合使用可增加DM，CP和必需FA TTR和大腿肌肉n-3 FA。然而，在饲喂亚麻籽的肉鸡中，补充Met或其组合会降低BW增益并对空肠形态产生负面影响。