Routine evaluation of the slaughter process is performed by the enumeration of the aerobic colony count, Enterobacteriaceae and Salmonella spp. on the carcass through destructive or non-destructive methods. With non-destructive methods, bacteria are counted from a minimum area of 100 cm² in different sampling sites on the pork carcasses, and the results of these investigated areas are pooled to one value for the complete carcass evaluation (a total of 400 cm²). However, the composition of the bacterial community present on the different sampling areas remains unknown. The aim of the study was to characterize the microbial population present on four areas (ham, back, jowl and belly) of eight pork carcasses belonging to two different slaughterhouses through culture-dependent (Matrix-assisted laser desorption/ionization time-of-flight Mass Spectrometry MALDI-TOF MS, combined with 16S rRNA gene sequencing) and complementary culture-independent (16S rRNA amplicon sequencing) methods. The presence of Salmonella spp. and Y. enterocolitica was additionally assessed. Using MALDI-TOF MS, Staphylococcus, Pseudomonas, and Escherichia coli were found to dominate the bacterial cultures isolated from the 8 carcasses. Based on the 16S rRNA amplicon sequencing analyses however, no specific genus clearly dominated the bacterial community composition. By using this culture-independent method, the most abundant genera in microbial populations of the ham, back, jowl and belly were found to be similar, but important differences between the two slaughterhouses were observed. Thus, present data suggests that the indigenous bacterial population of individual animals is overruled by the microbial population of the slaughterhouse in which the carcass is handled. Also, our data suggests that sampling of only one carcass area by official authorities may be appropriate for the evaluation of the hygienic status of the carcasses and therefore of the slaughter process.

屠宰过程的常规评估通过需氧菌落计数，肠杆菌科和沙门氏菌属的计数来进行。通过破坏性或非破坏性方法在胎体上 使用非破坏性方法，从猪肉car体上不同采样点的最小面积为100 cm ^2的细菌开始计数，并将这些调查区域的结果汇总为一个值，以进行完整的car体评估（总计400 cm ²）。但是，存在于不同采样区域的细菌群落的组成仍然未知。该研究的目的是通过依赖于培养物（基质辅助的激光解吸/电离飞行时间）来表征属于两个不同屠宰场的八个猪体的四个区域（火腿，背部，颈部和腹部）上的微生物种群质谱MALDI-TOF MS，结合16S rRNA基因测序）和非互补培养（16S rRNA扩增子测序）方法。沙门氏菌的存在。并进一步评估了小肠结肠炎耶尔森氏菌。使用MALDI-TOF MS，葡萄球菌，假单胞菌和大肠杆菌被发现主导了从8个屠体中分离出的细菌培养物。然而，基于16S rRNA扩增子测序分析，没有明确的属明确地主导细菌群落组成。通过使用这种与文化无关的方法，发现火腿，后背，披肩和腹部的微生物种群中最丰富的属相似，但是在两个屠宰场之间观察到了重要的区别。因此，目前的数据表明，个体动物的土著细菌种群被处理屠体的屠宰场的微生物种群所取代。同样，我们的数据表明，官方机构仅对一个屠体区域进行采样可能适合评估屠体的卫生状况，从而评估屠宰过程。