ABSTRACT Grape seed extract (GSE) is a proanthocyanidin-rich plant-derived agents and have been shown to increase dentin biomechanical properties and resistance of dentin collagen to degradation. High GSE concentrations had never been studied for bond strength (μTBS) maily in root canal dentin. This study investigated the effect of high concentrations of GSE in a shorter clinical time on the bond strength of fiberglass posts through the application of Universal adhesive in two modes (etch-and-rinse and self-etching). Seventy-two single-rooted bovine teeth were prepared and divided randomly into six groups (n=12) according to surface treatment and application mode of Universal adhesive. Two groups were etched with 37% phosphoric acid to expose collagen fibrils and a biomodification agent was applied next at two different higher concentrations (30% and 50% GSE), for 1 min. For the other two groups, GSE was previously applied and after Single Bond adhesive was used in self-etching system mode. The other sample were divided in two groups without GSE application, in the total-etch and self-conditioning of universal adhesive. Then, the fiber posts were enveloped with composite resin and cemented with RelyX Ultimate. The samples were sectioned in discs with thickness of 1 mm and submitted to the push-out test at 0.5 mm/min. Two teeth from each group were prepared for confocal electron microscopy to understand the adhesive behavior on dentin biomodified by GSE. The data presented homogeneity and were statistically analyzed using two-way ANOVA and Bonferroni’s complementary analysis (α=0.01). The 50% GSE used with the self-etching system maintained the μTBS values when compared to its control group (p>0.0172). The values of etch-and-rinse groups reduced after using 30% and 50% GSE (p

中文翻译：

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使用不同模式的通用粘合剂，较高的交联浓度是否会影响玻璃纤维柱的粘合强度？

摘要 葡萄籽提取物 (GSE) 是一种富含原花青素的植物源性物质，已被证明可以增加牙本质生物力学特性和牙本质胶原蛋白对降解的抵抗力。从未研究过高 GSE 浓度的根管牙本质粘合强度 (μTBS)。本研究通过以两种模式（蚀刻和冲洗和自蚀刻）应用通用粘合剂，研究了在更短的临床时间内高浓度 GSE 对玻璃纤维桩粘合强度的影响。制备72颗单根牛牙，根据万能胶的表面处理和应用方式随机分为6组（n=12）。两组用 37% 磷酸蚀刻以暴露胶原纤维，然后以两种不同的更高浓度（30% 和 50% GSE）应用生物改性剂，持续 1 分钟。对于其他两组，先使用 GSE，然后在自蚀刻系统模式下使用单键粘合剂。另一个样品分为两组，没有应用 GSE，在通用粘合剂的总蚀刻和自我调节中。然后，纤维柱用复合树脂包裹并用 RelyX Ultimate 粘合。将样品切成 1 毫米厚的圆盘，并以 0.5 毫米/分钟的速度进行推出测试。每组取两颗牙齿进行共聚焦电子显微镜检查，以了解 GSE 生物改性牙本质上的粘附行为。数据呈现同质性，并使用双向方差分析和 Bonferroni 互补分析 (α=0.01) 进行统计分析。与其对照组相比，与自蚀刻系统一起使用的 50% GSE 保持了 μTBS 值（p>0.0172）。使用 30% 和 50% GSE 后，蚀刻和冲洗组的值降低（p