As efforts to move a renewable economy grow, it will be necessary to make use of microbial conversion strategies for the production of novel materials or the upgrading of waste to high-value products. One critical technical challenge currently limiting waste upgrading remains the difficulty in obtaining single-pot conversion techniques where physical, chemical, and biological conversion are performed in a single step. To overcome this challenge, a detailed understanding of how different stresses impact microbial membrane stability will be necessary. Using all-atom molecular dynamics simulations, we examine the impacts of moderate concentrations of NaCl and CaCl₂on a model yeast plasma membrane. Weak, though statistically significant, changes in membrane morphology and dynamics functions are observed that are consistent with swelling and stiffening. Additionally, an examination of the ion-lipid contacts and the behavior of water at the water-membrane interface suggests that the impacts of these common salts may, in part, be mediated through changes to water-membrane hydrogen-bonding and hydration water dynamics.

随着推动可再生经济的发展，有必要利用微生物转化策略来生产新材料或将废物升级为高价值产品。目前限制废物升级的一项关键技术挑战仍然是难以获得在单个步骤中进行物理、化学和生物转化的单锅转化技术。为了克服这一挑战，需要详细了解不同压力如何影响微生物膜稳定性。使用全原子分子动力学模拟，我们检查了中等浓度的 NaCl 和 CaCl _{2 的影响}在模型酵母质膜上。虽然在统计上显着，但观察到膜形态和动力学函数的微弱变化，这与膨胀和硬化相一致。此外，对离子-脂质接触和水-膜界面处水的行为的检查表明，这些常见盐的影响可能部分是通过水-膜氢键和水合水动力学的变化来介导的。