A putative Solanum lycopersicum epoxide hydrolase, SlEH2, was discovered based on computer-aided analysis. Then, its encoding gene (sleh2) was expressed in E. coli BL21(DE3). The substrate spectrum assay exhibited that E. coli/sleh2, an E. coli transformant expressing SlEH2, possessed the activity of 74.8 U/g wet cell and enantiomeric ratio (E) of 145 towards racemic (rac-) para-chlorostyrene oxide (4a). The scale-up kinetic resolution of 400 mM rac-4a was conducted using 200 mg/mL wet cells of E. coli/sleh2 in the presence of 1% (v/v) Tween-20, retaining (R)-4a with 98.4% ee_s and 47.1% yield_s while affording (R)-4b with 92.0% ee_p and 49.5% yield_p.

基于计算机辅助分析，发现了推定的茄属茄科环氧化物水解酶S1 EH2。然后，其编码基因（sleh2）在大肠杆菌BL21（DE3）中表达。基板光谱测定显示出的是大肠杆菌/ sleh2，一个大肠杆菌转化体表达S1中EH2，具有74.8 U / g湿细胞和对映体比率（活性Ë朝向外消旋的145）（外消旋- ）对-chlorostyrene氧化物（4a）。400 mM rac - 4a的放大动力学分辨率使用200毫克/毫升的湿细胞中进行的大肠杆菌/ sleh2在1％的存在（v / v）的吐温20，保持（[R ）-图4a与98.4％ee值_小号和47.1％的产率_小号而得到（ř） -图4b与92.0％ee值_p和49.5％的产率_p。