Purpose

In this study, we report the prevalence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) amongst clinical specimens of patients suspected for sexually transmitted infections received at our laboratory and in addition report the prevalence of resistance-associated mutations (RAM) for ciprofloxacin in NG and azithromycin and moxifloxacin in MG.

Methods

All specimens received from December 2018 to May 2019 were tested for the four pathogens. In addition, the presence of RAM associated with resistance to ciprofloxacin in NG and to azithromycin and moxifloxacin in MG was determined by different real-time PCR assays on all NG- and MG-positive specimens.

Results

CT was detected most often (267/2613, 10.2%), followed by MG (106/2592, 4.1%), NG (41/2613, 1.6%) and TV (10/2592, 0.4%) amongst all specimens. The prevalence of ciprofloxacin RAM in NG was 21.2%, and the prevalence of RAM in MG was 40.6% for azithromycin and 8.1% for moxifloxacin. Nearly all specimens containing moxifloxacin-resistant MG also contained azithromycin-resistant MG.

Conclusion

CT is found most often in our population followed by MG and NG. By using molecular assays to detect RAM supplementary to pathogen identification of NG and MG, optimal therapy can be advised.

中文翻译：

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沙眼衣原体、淋病奈瑟菌、生殖支原体和阴道毛滴虫的流行率，包括在荷兰一个中心的相关耐药相关突变

目的

在这项研究中，我们报告了在我们实验室收到的疑似性传播感染患者的临床标本中沙眼衣原体(CT)、淋病奈瑟菌(NG)、生殖支原体(MG) 和阴道毛滴虫(TV)的流行情况，此外报告了环丙沙星在 NG 中和阿奇霉素和莫西沙星在 MG 中的耐药相关突变 (RAM) 的流行。

方法

从 2018 年 12 月至 2019 年 5 月收到的所有标本都进行了四种病原体的检测。此外，通过对所有 NG 和 MG 阳性标本进行不同的实时 PCR 检测，确定了 RAM 的存在与 NG 中对环丙沙星的耐药性以及 MG 中对阿奇霉素和莫西沙星的耐药性相关。

结果

在所有标本中最常检测到 CT (267/2613, 10.2%)，其次是 MG (106/2592, 4.1%)、NG (41/2613, 1.6%) 和 TV (10/2592, 0.4%)。NG中环丙沙星RAM的患病率为21.2%，MG中RAM的患病率为阿奇霉素40.6%和莫西沙星8.1%。几乎所有含有耐莫西沙星 MG 的标本也含有耐阿奇霉素 MG。

结论

CT 在我们的人群中最常见，其次是 MG 和 NG。通过使用分子检测来检测 RAM，补充 NG 和 MG 的病原体鉴定，可以建议最佳治疗。