Abstract

In this article the image processing of workflow from raw camera frames is detailed. The results of the visualization and quantitative analysis of the images from the rhodamine 6 G solution obtained using total internal reflection fluorescence microscopy are presented. The main stages for image processing of fluorescent molecules are considered. The following actions are described: acquisition of raw data, noise reduction, segmentation of areas of interest by characteristic features, localization of potential fluorophores, postprocessing (assessment of area parameters). Spatial information (time dependence of relative movement of the molecule positions, the concentration distribution depending on the wavelength) is obtained. These results confirm the possibility of applying computer analysis to study the nature of the movement of molecules in a system, their interactions, and dynamic properties of various molecular systems.

中文翻译：

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罗丹明6G溶液的分子动力学由荧光显微镜图像的计算机处理揭示。

摘要

本文详细介绍了从原始相机帧对工作流进行图像处理的过程。介绍了使用全内反射荧光显微镜从罗丹明6 G溶液中获得的图像的可视化和定量分析结果。考虑了荧光分子图像处理的主要阶段。描述了以下动作：原始数据的获取，降噪，通过特征划分感兴趣区域，定位潜在的荧光团，后处理（评估区域参数）。获得空间信息（分子位置相对运动的时间依赖性，取决于波长的浓度分布）。