Abstract

We develop an assay for the total content of xenobiotics from the polyaromatic hydrocarbon (PAH) and organochlorine pesticide (OCP) groups in samples of surface water containing suspended matter. It is found that the suspended matter isolated from water by filtration during sample preparation according to standard method ISO 5667:2006 may contain PAH and OCP xenobiotics. The xenobiotics containing in the filtrate and suspended matter isolated from water samples are extracted with n-hexane. After the extracts are concentrated by a factor of 250–1000, their qualitative and quantitative composition is determined by high-performance liquid phase chromatography with fluorescence detection and by gas chromatography mass spectrometry. For water samples, the limit of quantitation (LOQ) for OCP and PAH xenobiotics is found to be 0.1 and 0.005 μg/L, respectively, and in case of the suspended matter samples the LOQs are 0.1 and 0.005 ng/g. The determination error is expressed as the relative standard (mean-square) deviation S_r (%), and the completeness of xenobiotic extraction is expressed in per cents (r, %). The method is tested on samples of surface waters containing suspended matter in the range of 90 ± 10 to 2800 ± 50 mg/L. The developed method enables us to detect trace amounts of xenobiotics in water and in suspended matter in it. The method can be useful in studies of surface waters and in forecasting the level of contamination of aquatic environments with PAHs and OCPs released as a result of degradation of suspended matter that binds the xenobiotics.

中文翻译：

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地表水中多环芳烃和有机氯农药的定量

摘要

我们开发了一种用于测定含有悬浮物的地表水样品中的多环芳烃（PAH）和有机氯农药（OCP）组异生物素总含量的测定方法。已发现，按照标准方法ISO 5667：2006在样品制备过程中通过过滤从水中分离出的悬浮物可能含有PAH和OCP异种生物。萃取液中分离出的滤液和悬浮物中所含的异生物素用n萃取正己烷。提取物浓缩至250–1000倍后，可通过具有荧光检测功能的高效液相色谱和气相色谱质谱法确定其定性和定量组成。对于水样品，发现OCP和PAH外源生物的定量限（LOQ）分别为0.1和0.005μg/ L，对于悬浮物样品，LOQ为0.1和0.005 ng / g。测定误差以相对标准（均方）偏差S _r（％）表示，异生素提取的完整性以百分比表示（r，％）。该方法在含有90±10至2800±50 mg / L范围内悬浮物的地表水样品上进行了测试。先进的方法使我们能够检测到水中和水中悬浮物中的痕量异种生物。该方法可用于研究地表水和预测由于结合异生物素的悬浮物降解而释放出的PAH和OCP对水生环境的污染程度。