Homologous feeder culture system can efficiently promote the proliferation of embryonic germ (EG) cells or embryonic stem (ES) cells while avoiding contamination by exogenous proteins and pathogens. In this study, we compared the potency of using homologous porcine embryonic fibroblasts (PEFs), gonadal stromal cells (GSCs), porcine adipose–derived stem cells (PASCs), or porcine amniotic fluid stem (PAFS) cells as feeder cells for porcine EG growth, with the commonly used mouse embryonic fibroblasts (MEFs). We compared the feeder cell growth rates; secretion of growth factors including stem cell factor (SCF), basic fibroblast growth factor (bFGF), and leukemia inhibitory factor (LIF); the effects of growth factors on porcine PGC growth; and EG growth rates when individual cells were used as feeders. Our results showed that feeder cells secreted limited amounts of growth factors, and supplementation of growth factors can significantly improve the formation of EG colonies and number of passages (P < 0.05). GSC and PEF were more suitable for EG growth because of their faster growth rate and their support on EG growth. In conclusion, this study identified novel homologous cells that can be used for EG production.

同源饲养层培养系统可以有效促进胚胎胚芽（EG）细胞或胚胎干细胞（ES）的增殖，同时避免外源性蛋白质和病原体的污染。在这项研究中，我们比较了使用同源猪胚胎成纤维细胞 (PEF)、性腺基质细胞 (GSC)、猪脂肪干细胞 (PASC) 或猪羊水干细胞 (PAFS) 作为猪 EG 饲养细胞的效力与常用的小鼠胚胎成纤维细胞（MEF）一起生长。我们比较了饲养细胞的生长速度；生长因子的分泌，包括干细胞因子 (SCF)、碱性成纤维细胞生长因子 (bFGF) 和白血病抑制因子 (LIF)；生长因子对猪PGC生长的影响；当单个细胞用作饲养者时的 EG 生长速率。P < 0.05)。GSC 和 PEF 更适合 EG 生长，因为它们的生长速度更快并且对 EG 生长的支持。总之，本研究确定了可用于 EG 生产的新型同源细胞。