Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25 plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and cross-incompatibility) and post-zygotic (male sterility and inviability and weakness of F₁ hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises.

野土豆（茄属sp。）是马铃薯育种所需基因的重要储存库。它们大多数是二倍体和专性异型杂交，有性繁殖和无性繁殖。天然种群的样本在种质库中作为基本和应用末端的原位保存为种质，并在种子数量，种子活力或两者均降低时进行再生。在目前的种子再生方案中，没有考虑到内部繁殖障碍内的可能作用，在该方案中，要培育20至25株植物用于杂交工作。尽管如此，如果内部生殖屏障起作用，则每个种子再生周期中祖细胞的有效数量（Ne）可能会相对于植物的实际数量（N）有所减少。这个过程最终会导致基因侵蚀。分别在单个植物和基因型组合水平上研究了沙门氏菌的苦味，花粉的活力和大小，以及花粉与雌蕊的关系以及种子/果实的数量和类型（根据胚乳发育）。两种类型的障碍，合子前（花粉雌蕊自身和交叉不相容）和合子后（男性不育，F _1的成虫和弱势）在三个生长季节中检测到杂种），这表明并非该种的所有基因型都对下一代产生了相同的等位基因贡献。这些结果对于野生马铃薯和其他农作物野生近缘种质库管理者应该是一个警觉。由于种质库的目的是自然遗传多样性的异地维持，因此需要根据遗传前提来设计策略。