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Denaturing gradient gel electrophoresis and multi-SIR profiles of soil microbial communities from a karst doline at Aggtelek National Park, Hungary
Folia Microbiologica ( IF 2.4 ) Pub Date : 2020-10-08 , DOI: 10.1007/s12223-020-00828-y
Márton Mucsi 1, 2 , Gergely Krett 2 , Tibor Szili-Kovács 1 , János Móga 3 , Andrea K Borsodi 2, 4
Affiliation  

Soils play an important role in the ecosystem of karstic landscapes both as a buffer zone and as a source of acidity to belowground water. Although the microbiota of karstic soils is known to have a great effect on karstification processes, the activity and composition of these communities are largely unknown. This study gives a comparative analysis of soil microbial profiles from different parts of a doline located at Aggtelek, Hungary. The aim was to reveal the relationships between the vegetation type and genetic fingerprints and substrate utilisation (multi-SIR) profiles of the soil microbiota. Soil samples were collected in early and late springs along a transect in a doline covered with different types of vegetation. Genetic fingerprints of bacterial communities were examined by denaturing gradient gel electrophoresis (DGGE) based on the 16S rRNA gene, along with multi-SIR profiles of the microbial communities measured by the MicroResp method using 15 different carbon sources. Genetic fingerprinting indicated that vegetation cover had a strong effect on the composition of soil bacterial communities. Procrustean analysis showed only a weak connection between DGGE and multi-SIR profiles, probably due to the high functional redundancy of the communities. Seasonality had a significant effect on substrate usage, which can be an important factor to consider in future studies.



中文翻译:

匈牙利阿格泰莱克国家公园岩溶漏斗中土壤微生物群落的变性梯度凝胶电泳和多 SIR 图谱

土壤在岩溶景观的生态系统中发挥着重要作用,既是缓冲区,也是地下水酸度的来源。虽然已知岩溶土壤的微生物群对岩溶过程有很大影响,但这些群落的活动和组成在很大程度上是未知的。本研究对位于匈牙利 Aggtelek 的一条漏斗不同部分的土壤微生物特征进行了比较分析。目的是揭示植被类型与土壤微生物群的遗传指纹和底物利用 (multi-SIR) 剖面之间的关系。土壤样品在早春和晚春沿一条覆盖着不同类型植被的漏斗中的横断面采集。通过基于 16S rRNA 基因的变性梯度凝胶电泳 (DGGE) 以及通过 MicroResp 方法使用 15 种不同碳源测量的微生物群落的多 SIR 谱来检查细菌群落的遗传指纹。遗传指纹表明植被覆盖对土壤细菌群落的组成有很大影响。Procrustean 分析显示 DGGE 和多 SIR 配置文件之间只有微弱的联系,可能是由于社区的高功能冗余。季节性对底物使用有显着影响,这可能是未来研究中需要考虑的重要因素。遗传指纹表明植被覆盖对土壤细菌群落的组成有很大影响。Procrustean 分析显示 DGGE 和多 SIR 配置文件之间只有微弱的联系,可能是由于社区的高功能冗余。季节性对底物使用有显着影响,这可能是未来研究中需要考虑的重要因素。遗传指纹表明植被覆盖对土壤细菌群落的组成有很大影响。Procrustean 分析显示 DGGE 和多 SIR 配置文件之间只有微弱的联系,可能是由于社区的高功能冗余。季节性对底物使用有显着影响,这可能是未来研究中需要考虑的重要因素。

更新日期:2020-10-08
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