Abstract

With the progress in tissue engineering, the need for deep understanding of the interaction between individual cells and cellular systems with biocompatible polymer scaffolds has become obvious. This requires data on the adhesion and proliferation of cells on the scaffolds. Environmental scanning electron microscopy makes it possible to increase the pressure and humidity in a microscope chamber, thereby bringing the experimental conditions close to environmental. Scanning electron microscopy study of dermal fibroblasts is illustrated by the example of several scaffolds of different morphologies, including sponges, films, and nonwoven materials based on poly(L-)lactide, polycaprolactone, and a poly(L-)lactide-co-polycaprolactone. The data obtained show that the proposed method is promising for studying cell structures on polymer scaffolds.

中文翻译：

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各种类型聚合物支架上的皮肤成纤维细胞的环境扫描电子显微镜

摘要

随着组织工程学的进步，对具有生物相容性聚合物支架的单个细胞与细胞系统之间相互作用的深入理解的需求变得显而易见。这需要有关支架上细胞粘附和增殖的数据。环境扫描电子显微镜使增加显微镜腔室中的压力和湿度成为可能，从而使实验条件接近环境。皮肤成纤维细胞的扫描电子显微镜研究以不同形态的几种支架为例，包括海绵，薄膜和基于聚（L-丙交酯），聚己内酯和聚（L-丙交酯-共聚己内酯）的非织造材料。 。获得的数据表明，所提出的方法有望用于研究聚合物支架上的细胞结构。