Abstract

Cryo-electron tomography is a powerful tool for determining the three-dimensional structures of macromolecular complexes in their natural environment. The minimization of external impacts on the investigated objects, possibility of conducting in vitro and in cellulo experiments under close-to-native conditions, and high spatial resolution of the obtained three-dimensional reconstructions make cryo-electron tomography one of the most promising methods for studying a large class of objects in the fields of structural biology and visual proteomics. The main aspects of cryo-electron tomography are discussed as applied to studies of cell systems.

中文翻译：

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细胞系统的低温电子层析成像研究

摘要

低温电子断层扫描是确定自然环境中大分子复合物三维结构的有力工具。最大限度地减少对研究对象的外部影响，在接近自然条件下进行体外和纤维素实验的可能性以及获得的三维重建物的高空间分辨率，使得低温电子断层扫描成为最有希望的方法之一研究结构生物学和视觉蛋白质组学领域的一大类对象。讨论了冷冻电子断层扫描的主要方面，将其应用于细胞系统研究。